Localized Proton Magnetic Resonance Spectroscopy of Mouse Brain In Vivo at High Magnetic Field Strength

von Alireza Abaei Tafresh
Dissertation
Datum der mündl. Prüfung:2013-05-13
Erschienen:2014-10-01
Betreuer:Prof. Dr. Hans Hofsäss
Gutachter:Prof. Dr. Hans Hofsäss
Gutachter:PD Dr. Peter Dechent
Gutachter:Prof. Dr. Astrid Pundt
Gutachter:Prof. Dr. Jörg Enderlein
Gutachter:Prof. Dr. Stephan Waack
Gutachter:Prof. Dr. Carsten Damm
Zum Verlinken/Zitieren: http://dx.doi.org/10.53846/goediss-4709

 

 

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Zusammenfassung

Englisch

This work describes the implementation and optimization of single-voxel proton magnetic resonance spectroscopy for absolute quantification of regional metabolite concentration in mouse brain in vivo at 9.4 T. Optimized scheme of localized shimming, water suppression, and outer-volume-suppression enables a reliable detection of critical metabolite signals. Volume-of-interest localization technique is optimized for 10 different brain regions, where absolute concentrations of 16 different metabolites are determined. No partial volume effect of the cerebrospinal fluid is observed. Measurement of T1 and T2 relaxation times ensures an accurate quantification of metabolite concentrations with regard to the signal attenuation and the water content of brain tissue in vivo. Interestingly, the concentration of choline-containing compounds as well as of alanine turns out to be elevated in the striatum, while the concentration of lactate is elevated in the brainstem. High reproducibility of the method is ensured by the repeated measurement over four months. Relative detectabilities of strongly coupled metabolite resonances at 9.4 T are compared to those at 2.35 T.
Keywords: in vivo NMR spectroscopy; mouse brain; neurochemical profile
 
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