Localized Proton Magnetic Resonance Spectroscopy of Mouse Brain In Vivo at High Magnetic Field Strength

by Alireza Abaei Tafresh
Doctoral thesis
Date of Examination:2013-05-13
Date of issue:2014-10-01
Advisor:Prof. Dr. Hans Hofsäss
Referee:Prof. Dr. Hans Hofsäss
Referee:PD Dr. Peter Dechent
Referee:Prof. Dr. Astrid Pundt
Referee:Prof. Dr. Jörg Enderlein
Referee:Prof. Dr. Stephan Waack
Referee:Prof. Dr. Carsten Damm
Persistent Address: http://dx.doi.org/10.53846/goediss-4709

 

 

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Abstract

English

This work describes the implementation and optimization of single-voxel proton magnetic resonance spectroscopy for absolute quantification of regional metabolite concentration in mouse brain in vivo at 9.4 T. Optimized scheme of localized shimming, water suppression, and outer-volume-suppression enables a reliable detection of critical metabolite signals. Volume-of-interest localization technique is optimized for 10 different brain regions, where absolute concentrations of 16 different metabolites are determined. No partial volume effect of the cerebrospinal fluid is observed. Measurement of T1 and T2 relaxation times ensures an accurate quantification of metabolite concentrations with regard to the signal attenuation and the water content of brain tissue in vivo. Interestingly, the concentration of choline-containing compounds as well as of alanine turns out to be elevated in the striatum, while the concentration of lactate is elevated in the brainstem. High reproducibility of the method is ensured by the repeated measurement over four months. Relative detectabilities of strongly coupled metabolite resonances at 9.4 T are compared to those at 2.35 T.
Keywords: in vivo NMR spectroscopy; mouse brain; neurochemical profile
 
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