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dc.contributor.advisor Rizzoli, Silvio Prof. Dr.
dc.contributor.author Saka Kırlı, Sinem
dc.date.accessioned 2014-10-09T08:23:31Z
dc.date.available 2014-10-09T08:23:31Z
dc.date.issued 2014-10-09
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-0023-98FB-0
dc.language.iso eng de
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc 570 de
dc.title Studying Protein Organization in Cellular Membranes by High-Resolution Microscopy de
dc.type doctoralThesis de
dc.contributor.referee Kessel, Michael Prof. Dr.
dc.date.examination 2013-10-29
dc.description.abstracteng Most membrane proteins are found in clusters in the plasma membrane. For abundant proteins it is apparent that the clusters also tend to have patterned distributions, rather than being randomly scattered. To reveal the mechanism behind this observation, I have investigated all proteins in the plasma membrane simultaneously. This was achieved by large-scale metabolic labeling of proteins through incorporation of a non-canonical amino acid analogue and fluorescent tagging by click chemistry. Plasma membrane sheets were obtained from labeled PC12 and COS-7 cells and imaged by super-resolution stimulated emission depletion (STED) microscopy. Membrane proteins were found to form a mosaic like pattern. Heterogeneous domains, highly enriched in proteins, were distributed on a protein-poor background. I have termed these high-abundance domains “protein clouds” and assayed the contribution of different factors to their formation and maintenance. The protein cloud pattern was surprisingly robust and resisted to a variety of manipulations including changes in ionic composition and strength, decrease in protein density, disruption of cytoskeletal elements, and hydrolysis of phospholipids and sphingomyelin. Although actin disruption did not abolish the clouds, it resulted in formation of larger domains. Accordingly, actin was identified as a secondary factor that borders the clouds and prevents their coalescence. Depletion of cholesterol almost completely eliminated the protein clouds, in reversible fashion, suggesting that cholesterol is the major factor for the cloud patterning.  The cloud pattern was confirmed by two additional, independent techniques: first, label-free secondary ion mass spectrometry (SIMS) and second, STED fluorescence correlation spectroscopy (STED-FCS). For reliable application of SIMS, a novel experimental scheme, named correlated optical isotopic nanoscopy (COIN), was developed and verified.  To find out the relevance of clouds to specific proteins, distributions of different classes of proteins were investigated. All of the specific proteins analyzed were enriched in the protein clouds, but displayed differential enrichment profiles. Many proteins were preferentially located in particular areas, such as the edges or centers of the clouds. Some functional partners were observed to show similar profiles.  I conclude that enrichment of proteins in the clouds can act as a basic, low-hierarchy principle of membrane patterning, underlying the distributions of specific proteins. de
dc.contributor.coReferee Simons, Mikael Prof. Dr.
dc.contributor.thirdReferee Hell, Stefan Prof. Dr.
dc.contributor.thirdReferee Doenecke, Detlef Prof. Dr.
dc.contributor.thirdReferee Thumm, Michael Prof. Dr.
dc.subject.eng Protein Domains de
dc.subject.eng Nanodomains de
dc.subject.eng Multi-Protein Assemblies de
dc.subject.eng Protein Clouds de
dc.subject.eng Membrane Organization de
dc.subject.eng Correlated Optical Isotopic Nanoscopy de
dc.subject.eng COIN de
dc.subject.eng Secondary Isotope Mass Spectrometry de
dc.identifier.urn urn:nbn:de:gbv:7-11858/00-1735-0000-0023-98FB-0-4
dc.affiliation.institute Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) de
dc.subject.gokfull Biologie (PPN619462639) de
dc.identifier.ppn 798243597

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