GABA and glycine co-transmission in the developing mouse respiratory network

Rahman, Md Jamilur

dc.contributor.advisor	Hülsmann, Swen Prof. Dr.
dc.contributor.author	Rahman, Md Jamilur
dc.date.accessioned	2014-11-18T12:07:02Z
dc.date.available	2014-11-18T12:07:02Z
dc.date.issued	2014-11-18
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0023-993E-3
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4784
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	570	de
dc.title	GABA and glycine co-transmission in the developing mouse respiratory network	de
dc.type	doctoralThesis	de
dc.contributor.referee	Hülsmann, Swen Prof. Dr.
dc.date.examination	2014-04-02
dc.description.abstracteng	Inhibitory neurons in the pre-Bötzinger complex - a kernel for respiratory rhythm generation, are involved in the respiratory rhythm modulation. The co-transmission of GABA and glycine has been reported in different brain regions but not in the preBötC. The current study showed that GABA and glycine co-transmission is present in the neonatal mouse pre-Bötzinger complex. The co-transmission mediated inhibition was estimated up to 46.8% in the preBötC glycinergic neurons by the optimized “EPF” method based on decay kinetics of inhibitory postsynaptic currents. The co-transmission in the preBötC was further corroborated by single cell RT-PCR that showed 64% glycinergic neurons co-express GABAergic neuronal markers. The drastically reduced VIAAT expression in the preBötC and hypoglossal nucleus of the conditional VIAAT KO embryos strongly suggest that glycinergic neuronal marker (GlyT2) is co-expressed in most of GABAergic neurons in embryonic brainstem. The complete absence of sIPSCs in embryonic hypoglossal motoneurons further confirms wide expression of cre-recombinase among inhibitory neurons in a brainstem region which innervates hypoglossal nucleus. These results strongly suggest that GABA and glycine co-transmission could be ubiquitously present in the embryonic respiratory network and its distribution and relevance decreases during development. The cVIAAT KO embryos died due to respiratory failure resulting from complete loss of inhibitory transmission which is required for coupling between the preBötC and the pFRG to produce a normal respiratory rhythm. A disturbance in either glycine transmission or GABAergic transmission alone does not deteriorate the respiratory network immediately because the co-transmission of GABA and glycine provides a mechanism of compensation for loss of single inhibitory transmitter as gephyrin KO, GlyT2 KO and GAD67 mouse models survive longer than cVIAAT and VIAAT KO embryos. The cVIAAT KO embryos without any exception showed very common striking features like omphalocele, cleft palate and kyphotic posture which must be due to the loss of all inhibition but not with either GABAergic or glycinergic inhibition alone.	de
dc.contributor.coReferee	Heinrich, Ralf Prof. Dr.
dc.subject.eng	Respiratory network	de
dc.subject.eng	pre-Bötzinger complex	de
dc.subject.eng	co-transmission	de
dc.subject.eng	co-release	de
dc.subject.eng	GABA and glycine	de
dc.subject.eng	VIAAT	de
dc.subject.eng	GLYT2	de
dc.subject.eng	mIPSC	de
dc.subject.eng	mixed-mIPSC	de
dc.subject.eng	brainstem	de
dc.subject.eng	medulla	de
dc.subject.eng	Respiration	de
dc.subject.eng	Breathing	de
dc.subject.eng	Diaphragm	de
dc.subject.eng	Embryonic development	de
dc.subject.eng	Single cell PCR	de
dc.subject.eng	Immunostaining	de
dc.subject.eng	Reverse transcription PCR	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0023-993E-3-2
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.identifier.ppn	804314411

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Name:: PhD Dissertation_Md Jamilur ...
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Beschreibung:: PhD Dissertation

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GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1115]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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