Regulation des Sulfat-Anionen-Transporters-1, sat-1, in Caco2-Zellen durch Oxalat und dessen Vorstufen

Beck, Jan-Philipp

dc.contributor.advisor	Burckhardt, Birgitta-Christina Prof. Dr.
dc.contributor.author	Beck, Jan-Philipp
dc.date.accessioned	2014-11-25T11:35:47Z
dc.date.available	2014-12-15T23:50:07Z
dc.date.issued	2014-11-25
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0023-9940-C
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4790
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	610	de
dc.title	Regulation des Sulfat-Anionen-Transporters-1, sat-1, in Caco2-Zellen durch Oxalat und dessen Vorstufen	de
dc.type	doctoralThesis	de
dc.title.translated	Regulation of sulfate anion transporter-1, sat-1, in caco2 cells by oxalate and its precursors	de
dc.contributor.referee	Burckhardt, Birgitta-C. Prof. Dr.
dc.date.examination	2014-12-08
dc.description.abstracteng	Nephrolithiasis is a major health problem caused by hyperoxaluria. The sulfate anion transporter-1 which transports sulfate and oxalate might be involved in the development of hyperoxaluria, so further information concerning the influence of oxalate and its precursors on the expression of sat-1 is needed. Sat-1 expressing caco2 cells taken from a human adenocarcinoma were used to demonstrate the interaction of oxalate and its precursors glyoxylate, glycolate, glycine and hydroxyproline with sat-1. Caco2 cells were incubated with one of these substances for four days. After incubation changes in sat-1 mRNA expression were detected by using real-time polymerase chain reaction while protein expression of sat-1 was examined by Western blotting.Oxalate and its precursors glyoxylate, glycolate und hydroxyproline stimulated the expression of sat-1 mRNA while glycine did not. Oxalate and glyoxylate caused the most significant stimulation so that further examinations concerning the adaquate time of incubation and concentration of oxalate and glyoxylate were conducted.	de
dc.contributor.coReferee	Gross, Oliver Prof. Dr.
dc.subject.ger	Sulfat-Anionen-Transporter-1	de
dc.subject.ger	sat-1	de
dc.subject.ger	Caco2-Zellen	de
dc.subject.ger	Oxalat	de
dc.subject.ger	Hyperoxalurie	de
dc.subject.eng	sulfate anion transporter-1	de
dc.subject.eng	sat-1	de
dc.subject.eng	caco2 cells	de
dc.subject.eng	oxalate	de
dc.subject.eng	hyperoxaluria	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0023-9940-C-0
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.description.embargoed	2014-12-15
dc.identifier.ppn	805567984

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Human- und Zahnmedizin [2872]

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