Gesteigerte hippokampale Neurogenese nach experimenteller bakterieller Meningitis mit Streptococcus pneumoniae

Increased hippocampal neurogenesis after experimental bacterial meningitis with streptococcus pneumoniae

by Judith Bering
Doctoral thesis
Date of Examination:2014-12-08
Date of issue:2014-12-04
Advisor:Prof. Dr. Joachim Gerber
Referee:Prof. Dr. Joachim Gerber
Referee:PD Dr. Fred Lühder
Persistent Address: http://dx.doi.org/10.53846/goediss-4809

 

 

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Abstract

English

Bacterial meningitis remains a severe disease with high mortality rate. Despite of effective antibiotic therapy a lot of patients suffer from complications and long-term sequelae. The neuronal damage of the hippocampus is a well known attribute in bacterial meningitis – in animal model as well as in humans. The validation of increased neurogenesis after bacterial meningitis in animal model therefore represents an important observation. We investigate the apoptotic and neuroregenerative process in animals with induced bacterial meningitis by Streptococcus pneumoniae. We use two well established animal models – mouse and rabbit. The infected animals were compared with animals in a healty control group. The examined brain area is the dentate gyrus of hippocampus. The number of apoptotic cells is detected by In–Situ–Tailing, the amount of proliferating progenitor cells by BrdU-labeling. To confirm the neuronal origin of the new born cells, some slices are additionally marked by neuronal markers like MAP–2, TUC–4 and beta–tubulin. In both animal models we can show a higher proliferation rate of neuronal progenitor cells after bacterial meningitis, significant in rabbit model (p < 0.05, Mann–Whitney–U–Test). The results can be interpreted as an indication for existing potential for regeneration of central nervous system – especially dentate gyrus of hippocampus. The results of this study may support further research how to optimize influences to neurogenesis, evaluate new therapeutic strategies to reduce neuronal damage and help in better understanding the pathophysiology of bacterial meningitis with its wide range of mechanisms of regulations.
Keywords: neurogenesis; hippocampus; dentate gyrus; bacterial meningitis; pneumococcal meningitis
Schlagwörter: Neurogenese; Hippokampus; Gyrus dentatus; bakterielle Meningitis; Pneumokokkenmeningitis
 
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