From Slow to Ultra-fast MAS: Structural Determination of Type-Three Secretion System Bacterial Needles and Inorganic Materials by Solid-State NMR

Demers, Jean-Philippe

dc.contributor.advisor	Lange, Adam Dr.
dc.contributor.author	Demers, Jean-Philippe
dc.date.accessioned	2014-12-16T10:23:40Z
dc.date.available	2014-12-16T10:23:40Z
dc.date.issued	2014-12-16
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0023-9968-3
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-4656
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject.ddc	530	de
dc.title	From Slow to Ultra-fast MAS: Structural Determination of Type-Three Secretion System Bacterial Needles and Inorganic Materials by Solid-State NMR	de
dc.type	doctoralThesis	de
dc.contributor.referee	Samwer, Konrad Prof. Dr.
dc.date.examination	2014-04-23
dc.subject.gok	Physik (PPN621336750)	de
dc.description.abstracteng	This work on solid-state NMR methodologies and applications is divided in three parts. In the first part, three methods for solid-state NMR at ultra-fast Magic-Angle Spinning (MAS) are introduced: completely low-power protein assignment experiments, broadband low-power cross-polarization, sensitivity enhancement by magnetization recovery. The second part present studies of inorganic materials where 11 compounds containing silicon, phosphorus and tin atoms are characterized. A new method employing ultra-fast Magi-Angle Spinning for the study of compounds with extremely large Chemical Shift Anisotropy (CSA) is introduced. The third part consists in the study of the needle of bacterial Type-Three Secretion Systems (T3SS). Following the study of the T3SS needle of Shigella flexneri, a common architecture for T3SS needles is identified. A novel hybrid structure determination approach combining solid-state NMR and cryo-electron microscopy was employed to produce the atomic-resolution structure of the T3SS needle of Shigella flexneri and resolve a controversy in the field of Type-Three secretion.	de
dc.contributor.coReferee	Griesinger, Christian Prof. Dr.
dc.contributor.thirdReferee	Grubmüller, Helmut Prof. Dr.
dc.contributor.thirdReferee	Hofsäss, Hans Christian Prof. Dr.
dc.contributor.thirdReferee	Zweckstetter, Markus Prof. Dr.
dc.subject.eng	Solid-state NMR	de
dc.subject.eng	NMR pulse sequences	de
dc.subject.eng	Inorganic chemistry	de
dc.subject.eng	Type-Three Secretion System	de
dc.subject.eng	Protein structure	de
dc.subject.eng	Ultra-fast Magic-Angle Spinning	de
dc.subject.eng	Shigella flexneri	de
dc.subject.eng	Cryo-Electron Microscopy	de
dc.subject.eng	Paramagnetic doping	de
dc.subject.eng	Cross-polarization	de
dc.subject.eng	Low-power radio-frequency pulses	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0023-9968-3-6
dc.affiliation.institute	Fakultät für Physik	de
dc.identifier.ppn	812846982

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