Klonierung und funktionelle Charakterisierung des pOAT1 in ok-Zellen

Sendler, Mark Florian

dc.contributor.advisor	Hagos, Yohannes Prof. Dr.
dc.contributor.author	Sendler, Mark Florian
dc.date.accessioned	2015-11-25T09:49:52Z
dc.date.available	2015-12-02T23:50:05Z
dc.date.issued	2015-11-25
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0028-8641-A
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-5381
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-5381
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	610	de
dc.title	Klonierung und funktionelle Charakterisierung des pOAT1 in ok-Zellen	de
dc.type	doctoralThesis	de
dc.title.translated	Cloning and functional characterization of the pOAT1 in ok-cells	de
dc.contributor.referee	Bohnsack, Markus Prof. Dr.
dc.date.examination	2015-11-25
dc.description.abstracteng	We created and screened a pig kidney cDNA library for the organic anionen transporter 1 (pOAT1) und could isolate two splice variants (pOAT1: EMBL Acc. AJ308234 and pOAT1A: AJ308235). The functional characterization by expression of pOAT1 in Xenopus laevis oocytes and ok-cells showed an [3H]PAH uptake only for pOAT1. The apparrent Km for [3H]PAH in oocytes is 3,75 ± 1,6 µM and in ok-cells it is 8,54 ± 1,2 µM. This uptake was inhibited by 1mM probenecid and 1mM glutarate. The functional characterization revealed an similar cis inhibition of the pOAT1 mediated uptake for [3H]PAH by pyrazionat, urate, glutarate and probenicid as in pig BLMV. We also could show that the chlorid dependent uptake of [3H]PAH in pig BLMV is mediated by the pOAT1 whereas the chlorid independent urate uptake is mediated by the pOAT3. Testing steroids as substrate for the pOAT1 demonstrated a strong inhibition of the [3H]PAH uptake (Ki for estrone sulfate 113± 4,9µM; Ki for DHEA sulfate 116,5± 3,5µM) whereas [3H]estrone sulfate was not transported by the pOAT1.	de
dc.contributor.coReferee	Schön, Margarete Prof. Dr.
dc.subject.ger	pOAT1	de
dc.subject.ger	organische Anionen Transporter	de
dc.subject.ger	ok-Zellen	de
dc.subject.ger	slc22 Familie	de
dc.subject.ger	Schwein	de
dc.subject.eng	pOAT1	de
dc.subject.eng	organic anion transporter	de
dc.subject.eng	ok cells	de
dc.subject.eng	slc22 family	de
dc.subject.eng	pig	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0028-8641-A-6
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.description.embargoed	2015-12-02
dc.identifier.ppn	840882491

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Human- und Zahnmedizin [2872]

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