The role of the mir-310s in Hedgehog Signaling regulation under dietary stress in the Drosophila ovary

Çiçek, Ibrahim Ömer

dc.contributor.advisor	Shcherbata, Halyna PD Dr.
dc.contributor.author	Çiçek, Ibrahim Ömer
dc.date.accessioned	2016-02-03T09:20:53Z
dc.date.available	2016-02-03T09:20:53Z
dc.date.issued	2016-02-03
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-0028-86B2-A
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-5494
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	570	de
dc.title	The role of the mir-310s in Hedgehog Signaling regulation under dietary stress in the Drosophila ovary	de
dc.type	doctoralThesis	de
dc.contributor.referee	Dosch, Roland Dr.
dc.date.examination	2015-05-22
dc.description.abstracteng	Perturbations in nutrition and energy homeostasis can have deleterious effects on human health. Therefore, mechanisms controlling metabolic balance and initiating fast response to changing dietary conditions are essential. Such fast and robust responses at the cellular level can be achieved by microRNA (miRNA) mediated control of gene expression. In this study, the mir-310s are shown as important controllers of metabolic status of Drosophila. The mir-310s mutants have numerous starvation-sensitive genes deregulated at the protein and mRNA levels and exhibit diet-sensitive physiological phenotypes, such as low fecundity and high body fat accumulation. Moreover, the mir-310s regulate Hedgehog (Hh) signaling by targeting three Hh pathway-associated genes (Rab23, DHR96, and ttk), linking the egg production with the dietary status. In the ovary, this regulation is essential to tune the Hh signaling strength, which has to be kept at low levels under nutritional restriction. Here, Drosophila Rab23 is identified as a novel positive regulatory element of Hh signaling. Rab23 is shown to act cell-autonomously, regulating Hh ligand release by facilitating its intracellular mobility. These findings shed light on miRNA-based control of the Hh pathway in a nutrition-sensitive context. From flies to humans, the conservation of mir-310s seed sequences, Rab23, and Hh signaling raises the possibility that this control mechanism is existing in humans as well. Therefore, these findings are important for the better understanding of metabolic diseases and development of miRNA related therapeutics.	de
dc.contributor.coReferee	Wodarz, Andreas Prof. Dr.
dc.subject.eng	Drosophila melanogaster	de
dc.subject.eng	miRNA	de
dc.subject.eng	diet	de
dc.subject.eng	Rab23	de
dc.subject.eng	Hedgehog signaling	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-0028-86B2-A-7
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.identifier.ppn	847079848

Dateien

Name:CICEK I Omer Doctoral Thesis.pdf

Größe:4.612Mb

Format:PDF

Öffnen

Name:: CICEK I Omer Doctoral Thesis.pdf
Größe:: 4.612Mb
Format:: PDF

Öffnen

Das Dokument erscheint in:

GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1112]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

Zur Kurzanzeige