dc.contributor.advisor | Stadelmann-Nessler, Christine Prof. Dr. | |
dc.contributor.author | Behling, Felix | |
dc.date.accessioned | 2016-09-28T09:38:59Z | |
dc.date.available | 2016-10-04T22:50:05Z | |
dc.date.issued | 2016-09-28 | |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-002B-7C0F-5 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-5861 | |
dc.language.iso | eng | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.ddc | 610 | de |
dc.title | Identification of Prognostically Relevant Cellular Markers of Differentiation in Glioblastoma | de |
dc.type | doctoralThesis | de |
dc.contributor.referee | Kramm, Christof Prof. Dr. | |
dc.date.examination | 2016-09-27 | |
dc.description.abstracteng | Introduction:
Glioblastoma multiforme is the most common primary brain tumor. Despite intense
research efforts worldwide, the prognosis of patients diagnosed with this tumor remains poor. Several clinical and molecular parameters influence overall survival. However, apart from IDH-1, a prognostic marker that can be assessed immunohistochemically is still lacking. In this study the prognostic impact of several immunohistochemical markers was assessed in a cohort of 120 GBMs.
Materials and Methods:
In this study 120 tumor samples of patients diagnosed with glioblastoma multiforme
underwent tissue microarray processing and subsequent immunohistochemical staining. All patients received adjuvant radiation and chemotherapy with an alkylating
agent. The cellular expression of NogoA, OLIG2, GFAP, Ki67 and p53 and mutations
of IDH-1 were assessed regarding their impact on overall survival, age and clinical
status (KPS). Kaplan-Meier curve analysis (Gehan’s Wilcoxon test), the independent
two-sample t-test and multivariate analysis (Cox regression) were applied.
Results:
A small group of patients with low GFAP expression levels showed a tendency towards
younger age at the time of diagnosis, a finding yet not described in the literature. The
detection of IDH-1 mutations did not show a significant prognostic impact, but a clear
trend towards younger age (p=0.05063). Immunopositivity of p53 was significantly
associated with longer overall survival in the Kaplan-Meier analysis (p=0.0480) and
with younger age (p=0.04054) and better clinical status (p=0.01165). There was a
strong trend towards longer overall survival of patients with lower expression levels of
OLIG2, most pronounced at the cutoff at 30%, (p=0.0587). Interestingly, Cox regression analysis showed that expression rates of OLIG2 below
30% were a significant independent prognostic marker in glioblastoma multiforme
(p=0.0168). The presence of p53 immunopositivity did not reach statistical significance
in the multivariate analysis.
Conclusion:
In conclusion, the percentage of OLIG2 expressing tumor cells emerged as a prognostic factor for overall survival. Further studies are necessary to reveal its exact role in gliomagenesis. | de |
dc.contributor.coReferee | Oppermann, Martin Prof. Dr. | |
dc.subject.eng | Glioblastoma | de |
dc.subject.eng | Immunohistochemistry | de |
dc.subject.eng | Prognostic Markers | de |
dc.subject.eng | OLIG2 | de |
dc.subject.eng | P53 | de |
dc.subject.eng | NogoA | de |
dc.subject.eng | GFAP | de |
dc.subject.eng | Ki67 | de |
dc.subject.eng | Overall Survival | de |
dc.subject.eng | IDH1 | de |
dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-002B-7C0F-5-2 | |
dc.affiliation.institute | Medizinische Fakultät | de |
dc.subject.gokfull | Medizin (PPN619874732) | de |
dc.subject.gokfull | Histopathologie {Medizin} (PPN619875704) | de |
dc.description.embargoed | 2016-10-04 | |
dc.identifier.ppn | 869470264 | |