Transfer von Pluripotenzfaktoren maligner Stro-1-positiver und Stro-1 negativer Zellen auf tumorfremde somatische Zellen

Walter, Julia

dc.contributor.advisor	Wiese, Karl Günter Prof. Dr. Dr.
dc.contributor.author	Walter, Julia
dc.date.accessioned	2016-10-18T11:16:03Z
dc.date.available	2016-11-06T23:50:05Z
dc.date.issued	2016-10-18
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-002B-7C2D-1
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-5895
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	610	de
dc.title	Transfer von Pluripotenzfaktoren maligner Stro-1-positiver und Stro-1 negativer Zellen auf tumorfremde somatische Zellen	de
dc.type	doctoralThesis	de
dc.title.translated	Study on the transfer of pluripotency factors from malignant STRO-1+ and STRO-1− cells to non-tumorous somatic cells	de
dc.contributor.referee	Miosge, Nicolai Prof. Dr.
dc.date.examination	2016-10-25
dc.description.abstracteng	Despite the fact that a tremendous research effort has been made, the formation, dissemination and the progress of metastatic spread of a tumour disease is not yet entirely understood. However, it is known that so-called tumour stem cells, which share many similarities with true stem cells, play a vital role regarding tumour progression, malignity and the resistance to chemotherapy treatment of carcinosis. In this context, we identified in an ectodermal tumour cell line (Hep2, laryngeal carcinoma cells) and in a mesenchymal cell line (SAOS, osteosarcoma cells) a subpopulation, which expresses the stem cell marker STRO-1. Positive and negative STRO-1 cells were further studied with regard to the transfer of pluripotency factors, which are known to cause induced pluripotency in cells. Both cell lines express the pluripotency factors Oct4, c-myc, Sox2 and Nanog. In co-cultural trials, we were able to detect that all osteosarcoma cells (STRO-1+ and STRO-1−) had transferred the four pluripotency factors to somatic cells, in contrast to Hep2 cells, where only a transfer of Oct4 and Nanog was detected. The various intercellular junctions found in the co-culture led to the assumption of a transfer of these factors by means of nanotubes. A transfer of Oct4 by means of exosomes could be excluded. In summary, it was shown that both tumour cell lines were able to transfer pluripotency factors to somatic cells and consequently influence their cellular behaviour. However, it still needs to be fully clarified, which cell population is responsible for the transfer of these factors and how they eventually affect the somatic cells. Nevertheless, the results could be considered a point of reference regarding a mechanism for the expansion of a tumour cell subpopulation, which causes redifferentiation leading to a more aggressive tumour spread.	de
dc.contributor.coReferee	Oppermann, Martin Prof. Dr.
dc.subject.ger	Stro-1	de
dc.subject.ger	Oct4	de
dc.subject.ger	Pluripotenz	de
dc.subject.ger	Krebs	de
dc.subject.ger	SAOS	de
dc.subject.ger	Hep2	de
dc.subject.eng	Stro-1	de
dc.subject.eng	Oct4	de
dc.subject.eng	pluripotency	de
dc.subject.eng	cancer	de
dc.subject.eng	SAOS	de
dc.subject.eng	Hep2	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-002B-7C2D-1-8
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.subject.gokfull	Zahn-, Mund- und Kieferheilkunde - Allgemein- und Gesamtdarstellungen (PPN619876360)	de
dc.description.embargoed	2016-11-06
dc.identifier.ppn	870414305

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