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dc.contributor.advisor Wiese, Karl Günter Prof. Dr. Dr.
dc.contributor.author Walter, Julia
dc.date.accessioned 2016-10-18T11:16:03Z
dc.date.available 2016-11-06T23:50:05Z
dc.date.issued 2016-10-18
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-002B-7C2D-1
dc.language.iso deu de
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc 610 de
dc.title Transfer von Pluripotenzfaktoren maligner Stro-1-positiver und Stro-1 negativer Zellen auf tumorfremde somatische Zellen de
dc.type doctoralThesis de
dc.title.translated Study on the transfer of pluripotency factors from malignant STRO-1+ and STRO-1− cells to non-tumorous somatic cells de
dc.contributor.referee Miosge, Nicolai Prof. Dr.
dc.date.examination 2016-10-25
dc.description.abstracteng Despite the fact that a tremendous research effort has been made, the formation, dissemination and the progress of metastatic spread of a tumour disease is not yet entirely understood. However, it is known that so-called tumour stem cells, which share many similarities with true stem cells, play a vital role regarding tumour progression, malignity and the resistance to chemotherapy treatment of carcinosis. In this context, we identified in an ectodermal tumour cell line (Hep2, laryngeal carcinoma cells) and in a mesenchymal cell line (SAOS, osteosarcoma cells) a subpopulation, which expresses the stem cell marker STRO-1. Positive and negative STRO-1 cells were further studied with regard to the transfer of pluripotency factors, which are known to cause induced pluripotency in cells. Both cell lines express the pluripotency factors Oct4, c-myc, Sox2 and Nanog. In co-cultural trials, we were able to detect that all osteosarcoma cells (STRO-1+ and STRO-1−) had transferred the four pluripotency factors to somatic cells, in contrast to Hep2 cells, where only a transfer of Oct4 and Nanog was detected. The various intercellular junctions found in the co-culture led to the assumption of a transfer of these factors by means of nanotubes. A transfer of Oct4 by means of exosomes could be excluded. In summary, it was shown that both tumour cell lines were able to transfer pluripotency factors to somatic cells and consequently influence their cellular behaviour. However, it still needs to be fully clarified, which cell population is responsible for the transfer of these factors and how they eventually affect the somatic cells. Nevertheless, the results could be considered a point of reference regarding a mechanism for the expansion of a tumour cell subpopulation, which causes redifferentiation leading to a more aggressive tumour spread. de
dc.contributor.coReferee Oppermann, Martin Prof. Dr.
dc.subject.ger Stro-1 de
dc.subject.ger Oct4 de
dc.subject.ger Pluripotenz de
dc.subject.ger Krebs de
dc.subject.ger SAOS de
dc.subject.ger Hep2 de
dc.subject.eng Stro-1 de
dc.subject.eng Oct4 de
dc.subject.eng pluripotency de
dc.subject.eng cancer de
dc.subject.eng SAOS de
dc.subject.eng Hep2 de
dc.identifier.urn urn:nbn:de:gbv:7-11858/00-1735-0000-002B-7C2D-1-8
dc.affiliation.institute Medizinische Fakultät de
dc.subject.gokfull Medizin (PPN619874732) de
dc.subject.gokfull Zahn-, Mund- und Kieferheilkunde - Allgemein- und Gesamtdarstellungen (PPN619876360) de
dc.description.embargoed 2016-11-06
dc.identifier.ppn 870414305

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