Navigation ▼

Zur Kurzanzeige

dc.contributor.advisor Diederichsen, Ulf Prof. Dr.
dc.contributor.author Czerny, Florian
dc.date.accessioned 2016-11-17T09:17:08Z
dc.date.available 2016-11-17T09:17:08Z
dc.date.issued 2016-11-17
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-002B-7CAB-3
dc.language.iso eng de
dc.publisher Niedersächsische Staats- und Universitätsbibliothek Göttingen de
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc 540 de
dc.title Development of Zinc-Finger-Based Artificial Restriction Endonucleases and Fluorescent Peptidyl Metal Sensors de
dc.type doctoralThesis de
dc.contributor.referee Diederichsen, Ulf Prof. Dr.
dc.date.examination 2016-08-08
dc.description.abstracteng Zinc finger proteins are ubiquitous in the cellular environment having an excessive diversity and performing variable tasks. Their outstanding capability to develop a sequence-specific bond to deoxyribonucleic acid (DNA) makes them one of the most abundant DNA-binding domains present in many eukaryotic and prokaryotic transcription factors.  The first part of this thesis describes the modification of the zinc finger protein from Zif268 by introducing artificial dinuclear amino acids to establish novel and substantially downsized artificial restriction endonucleases. Furthermore, a phosphoserine residue was incorporated in the protein to study its recruiting capacity for an external Ce(IV)/EDTA complex representing the hydrolytically active species.  In the second part, the zinc finger’s overwhelming zinc coordination properties were used to develop fluorescent peptidyl metal sensors. This was achieved by exchanging a histidine residue, which is part of the metal coordination site of the protein, for a propargylglycine residue and subsequent attachment of the fluorophore by the means of a copper-induced azide alkyne cycloaddition. de
dc.contributor.coReferee Meyer, Franc Prof. Dr.
dc.contributor.thirdReferee Elmroth, Sofi Prof. Dr.
dc.subject.eng Zinc finger de
dc.subject.eng Zif268 de
dc.identifier.urn urn:nbn:de:gbv:7-11858/00-1735-0000-002B-7CAB-3-6
dc.affiliation.institute Fakultät für Chemie de
dc.subject.gokfull Chemie  (PPN62138352X) de
dc.identifier.ppn 872756718

Dateien

Das Dokument erscheint in:

Zur Kurzanzeige