Einfluss von LEF1 auf das Tumorwachstum im Burkitt-Lymphom-Xenograft-Modell

Einfluss von LEF1 auf das Tumorwachstum im Burkitt-Lymphom-Xenograft-Modell

Influence of LEF1 on tumor growth in a Burkitt-Lymphoma-Xenograft-Model

Stefan Ueberdiek

Doctoral thesis

Date of Examination: 2016-10-27

Date of issue: 2016-11-18

Advisor: Kube, Dieter Prof. Dr.

Referee: Kube, Dieter Prof. Dr.

Referee: Wilting, Jörg Prof. Dr.

Persistent Address: http://hdl.handle.net/11858/00-1735-0000-002B-7CB1-4

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Abstract

English

Because of the limited knowledge about the biology of the highly aggressive Burkitt- Lymphoma is still possible in many cases no cure, it is all the more important, Possible therapeutic approaches. One possible starting point is the Transcription factor LEF-1, which is aberrantly expressed in BL cells. But for that Still have many more insights about the function of LEF1 in Burkitt's lymphoma be won. In this work the further establishment and characterization of the LEF1-KD as well as the Scrb-shRNA-BL2 cell lines. Furthermore, the Xenograft model CAM assay as a suitable model for the study of LEF1-aberrant and LEF1-deficient Burkitt's lymphoma. With regard to the biological function of LEF1 in the BL2 cells, ovo Significantly smaller tumor areas of LEF1-deficient Burkitt's lymphomas compared to The LEF1-aberrant Burkitt's lymphomas. From this finding, the underlying tumor characteristics such as Growth of tumors and cell proliferation, That LEF1 has positively regulating influences on these aspects. More information Were found to have positive effects on LEF1 and migration Invasiveness of lymphoma cells. Because the tumor microenvironment, which is characterized by the MatriGel was able to improve significantly from LEF1-aberrant lymphoma cells Can be decomposed.

Keywords: LEF1; Burkitt-Lymphoma; CAM-Assay