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Synthesis and coordination chemistry of tetradentate chelators based on ligand-appended G-quadruplex structures

dc.contributor.advisorClever, Guido Prof. Dr.
dc.contributor.authorEngelhard, David Maximilian
dc.date.accessioned2016-12-12T10:36:17Z
dc.date.available2016-12-12T10:36:17Z
dc.date.issued2016-12-12
dc.identifier.urihttp://hdl.handle.net/11858/00-1735-0000-002B-7CD4-7
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-6035
dc.language.isoengde
dc.publisherNiedersächsische Staats- und Universitätsbibliothek Göttingende
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc540de
dc.titleSynthesis and coordination chemistry of tetradentate chelators based on ligand-appended G-quadruplex structuresde
dc.typedoctoralThesisde
dc.contributor.refereeClever, Guido Prof. Dr.
dc.date.examination2016-01-14
dc.description.abstractengIn this thesis, the concept of metal base-pairing was adapted for usage with the DNA secondary structure of G-quadruplexes. Apart from the well-known B-DNA double helix, oligonucleotides can form a variety of different topologies. G-quadruplexes self-assemble from guanine-rich DNA strands forming G-quartets, which stack on top of each other, and are believed to play an important biological role, e. g. during DNA transcription, replication and telomere maintenance. The unique folding behaviour of G-quadruplexes was used to establish a “metal base-tetrad” motif in analogy to the metal base-pairing concept developed for duplex DNA. The metal base-tetrad consists of four identical monodentate pyridine ligands, each covalently appended via an alkyl linker to a guanine-rich oligonucleotide strand, and a transition metal ion coordinating the ligands. Formation of the metal base-tetrad stabilises the respective G-quadruplex topology and is also able to trigger G-quadruplex formation. Characterisation was achieved by UV-VIS, CD, and EPR spectroscopy, ESI mass spectrometry, and gel electrophoresis. Three main results have been achieved. First, a simple but easily adaptable ligand synthesis was developed and the successful ligand incorporation into guanine-rich oligonucleotides was demonstrated. Second, stabilisation of tetramolecular G-quadruplexes, with even more than one instance of the metal base-tetrad could be achieved. Third, stabilisation and topology switching induced by transition metal ion coordination was shown for unimolecular G-quadruplexes.de
dc.contributor.coRefereeHöbartner, Claudia Prof. Dr.
dc.subject.engDNA nanotechnologyde
dc.subject.engsupramolecular chemistryde
dc.subject.engG-quadruplexesde
dc.subject.engMetal base-tetradde
dc.identifier.urnurn:nbn:de:gbv:7-11858/00-1735-0000-002B-7CD4-7-2
dc.affiliation.instituteFakultät für Chemiede
dc.subject.gokfullChemie  (PPN62138352X)de
dc.identifier.ppn874354641


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