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dc.contributor.advisor Wolff, Hendrik PD Dr.
dc.contributor.author Holzer, Claudia Anna
dc.date.accessioned 2017-02-20T09:52:05Z
dc.date.available 2017-03-20T23:50:07Z
dc.date.issued 2017-02-20
dc.identifier.uri http://hdl.handle.net/11858/00-1735-0000-002B-7D4F-B
dc.language.iso deu de
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc 610 de
dc.title Analyse der Expression von Chemokinen und Chemokinrezeptoren in HNO-Tumorzellen unter Radiochemotherapie de
dc.type doctoralThesis de
dc.title.translated Analysis of chemokine and chemokine receptor expression in squamous cell carcinoma of the head and neck cell lines de
dc.contributor.referee Canis, Martin Prof. Dr.
dc.date.examination 2017-03-13
dc.description.abstracteng As patients suffering from squamous cell carcinoma of the head and neck (SCCHN) commonly have a poor prognosis, there is a need to understand the impacts on the behaviour of tumor cells better. It is already known that the irridation of tumor cells leads to a modified expression of some chemokines and chemokine receptors. The purpose of this work was to analyze the expression of the chemokines CXCL12, CXCL1, CXCL3 and CCL20 and the chemokine receptors CXCR4, CXCR1, CCR6 as well as CCR7 in SCCHN lines under chemotherapy and radiochemotherapy. Therefore colony forming assays have been made with two normal cell lines and six SCCHN lines. In addition the expression of the above-mentioned chemokines and chemokine receptors was analyzed in the same cell lines by real-time PCR. For both examinations the cell lines were irridated and incubated with cisplatin. Afterwards single chemotherapy, single radiotherapy and combined radiochemotherapy were compared. Under single chemotherapy with cisplatin the surviving of all cell lines decreased significantly in comparison to the untreated cells. The effect was much more distinctive with the SCCHN lines than the normal cell lines. When compared, combined radiochemotherapy had only an additional effect, radiosensitization could not be detected. In most of the examined samples, there was a stronger reinforcement in radiochemotherapy -compared to single radiotherapy - regarding the modification of the expression of chemokines and chemokine receptors. The differing behaviour of the expression of chemokines and chemokine receptors under the various therapies shows the complexity of the issue. To fully understand the influence of chemokines and chemokine receptors on the behaviour of tumor cells and their use in clinical practice further studies need to be conducted. de
dc.contributor.coReferee Mausberg, Rainer Prof. Dr.
dc.subject.ger Chemokine de
dc.subject.ger Chemokinrezeptoren de
dc.subject.ger Radiochemotherapie de
dc.subject.ger Cisplatin de
dc.subject.ger CXCL12 de
dc.subject.ger CXCL1 de
dc.subject.ger CXCL3 de
dc.subject.ger CCL20 de
dc.subject.ger CXCR4 de
dc.subject.ger CXCR1 de
dc.subject.ger CCR6 de
dc.subject.ger CCR7 de
dc.subject.ger Koloniebildungsversuch de
dc.subject.ger Real-time PCR de
dc.subject.eng SCCHN de
dc.subject.eng Radiochemotherapy de
dc.subject.eng chemokines de
dc.subject.eng chemokine receptors de
dc.subject.eng CXCL12 de
dc.subject.eng CXCL1 de
dc.subject.eng CXCL3 de
dc.subject.eng CCL20 de
dc.subject.eng CXCR4 de
dc.subject.eng CXCR1 de
dc.subject.eng CCR6 de
dc.subject.eng CCR7 de
dc.subject.eng Cisplatin de
dc.subject.eng real-time PCR de
dc.subject.eng colony forming assays de
dc.identifier.urn urn:nbn:de:gbv:7-11858/00-1735-0000-002B-7D4F-B-7
dc.affiliation.institute Medizinische Fakultät de
dc.subject.gokfull Radiologie / Bildgebende Verfahren / Ultraschall / Nuklearmedizin / Strahlenschutz - Allgemein- und Gesamtdarstellungen (PPN619875585) de
dc.subject.gokfull Oto-Rhino-Laryngologie (PPN619876220) de
dc.description.embargoed 2017-03-20
dc.identifier.ppn 88053804X

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