Localizing and tracking of ﬂuorescent molecules with minimal photon ﬂuxes

Eilers, Yvan

dc.contributor.advisor	Hell, Stefan Prof. Dr.
dc.contributor.author	Eilers, Yvan
dc.date.accessioned	2018-01-19T09:44:03Z
dc.date.available	2018-01-19T09:44:03Z
dc.date.issued	2018-01-19
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-002E-E329-2
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-6540
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-6540
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	530	de
dc.title	Localizing and tracking of ﬂuorescent molecules with minimal photon ﬂuxes	de
dc.type	doctoralThesis	de
dc.contributor.referee	Köster, Sarah Prof. Dr.
dc.date.examination	2017-02-07
dc.subject.gok	Physik (PPN621336750)	de
dc.description.abstracteng	In this thesis MINFLUX is presented, a novel optical scheme to determine the position of individual photon emitters in space. By probing the emitter with adapted illumination proﬁles featuring an intensity zero, the number of emitted photons needed for precise localization can be minimized. Proof-of-concept measurements on static emitters reduced the number of photon detections by 22-fold at equal localization precision compared to widespread camera-based methods. Localization precisions of 2.5 nanometer were realized at 400 microsecond time resolution using only 100 photons. With MINFLUX, the temporal resolution in single molecule tracking experiments of mEos2 fused to 30S ribosomal subunits could be increased by 100-fold. Using the available photons more eﬀectively, the number of localizations was enhanced by more than an order of magnitude permitting a 3-fold improvement in the diﬀusion coeﬃcient estimation precision. Theoretical evaluations showed that it can be expected that future MINFLUX implementations will improve the spatio-temporal resolution beyond the presented experimental results and thus further facilitate the study of fundamental processes in living organisms at their characteristic time and length scales.	de
dc.contributor.coReferee	Ropers, Claus Prof. Dr.
dc.subject.eng	Single molecule tracking	de
dc.subject.eng	MINFLUX	de
dc.subject.eng	Superresolution microscopy	de
dc.subject.eng	Nanoscopy	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E329-2-2
dc.affiliation.institute	Fakultät für Physik	de
dc.identifier.ppn	1011237547

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Fakultät für Physik (inkl. GAUSS) [926]

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