Vergleich der antibakteriellen Effektivität vier unterschiedlicher Techniken zur Aktivierung der Wurzelkanalspülung  (Hand, Ultraschall, RinsEndo®, EndoVac®)  auf Enterococcus faecalis anhand eines Wurzelkanal-Biofilm-Modells

Eberl, Monika Diana

dc.contributor.advisor	Rödig, Tina PD Dr.
dc.contributor.author	Eberl, Monika Diana
dc.date.accessioned	2018-01-24T09:52:18Z
dc.date.available	2018-02-27T23:50:44Z
dc.date.issued	2018-01-24
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-002E-E32F-5
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-6695
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	610	de
dc.title	Vergleich der antibakteriellen Effektivität vier unterschiedlicher Techniken zur Aktivierung der Wurzelkanalspülung (Hand, Ultraschall, RinsEndo®, EndoVac®) auf Enterococcus faecalis anhand eines Wurzelkanal-Biofilm-Modells	de
dc.type	doctoralThesis	de
dc.title.translated	Comparison of the antibacterial effectiveness of four different endodontic irrigation techniques (conventional syringe irrigation, passive ultrasonic irrigation, EndoVac® irrigation, RinsEndo® irrigation) against Enterococcus faecalis on the basis of an intracanal biofilm model	de
dc.contributor.referee	Rödig, Tina PD Dr.
dc.date.examination	2018-02-20
dc.description.abstracteng	OBJECTIVE: The failure of primary root canal treatments is mostly due to persisting bacteria. Several irrigation techniques have been introduced with the main objective of improving root canal disinfection. The aim of this in vitro study was the comparison of the effectiveness of four different endodontic irrigation techniques (conventional syringe irrigation, passive ultrasonic irrigation, EndoVac® irrigation, RinsEndo® irrigation) in reducing intraradicular Enterococcus faecalis biofilms. METHODS: 70 extracted single-rooted human teeth were used for the in-vitro model. After a standardized root canal preparation the smear layer was removed with EDTA 17% and NaOCl 1%. Distribution to a total of eight experimental groups was made: two groups (each n = 5) served for analyzing the smear layer removal (SL) and the grown biofilm (BF) by means of scanning electron microscopy. Four groups (each n = 10) served for analyzing the four irrigation techniques. Furthermore, a positive and a negative control group (each n = 10) were analyzed. An E. faecalis biofilm was grown in the root canals of the conventional syringe irrigation, passive ultrasonic irrigation, EndoVac® irrigation, RinsEndo® irrigation, positive control and BF groups for 72 hours with repeated injection of a fresh standardized E. faecalis solution after 24 and 48 hours. Those groups were then disinfected with 6 ml of NaOCl 1% for 3 Minutes, with the exception of the RinsEndo® system which used about 18 ml of NaOCl 1%. Dentin particles were removed with endodontic instruments and incubated on an agar culture medium. After 24 hours the colony forming units (CFU) were counted and statistically evaluated. RESULTS: The removal of the smear layer and the growth of a biofilm were confirmed by means of the SEM analysis. All irrigation techniques significantly reduced the number of E. faecalis in root canals (p < 0,0001). No disinfection measure led to a complete elimination of E. faecalis from the root canal system. There was no significant difference in the effectiveness of the four different endodontic irrigation techniques. CONCLUSION: There was no evident antibacterial superiority of any of the four irrigation techniques evaluated in the present in vitro study. The use of an effective antibacterial rinsing solution like NaOCl 1% seems to be most important.	de
dc.contributor.coReferee	Sennhenn-Kirchner, Sabine PD Dr.
dc.subject.eng	sodium hypochlorite	de
dc.subject.eng	EndoVac	de
dc.subject.eng	RinsEndo	de
dc.subject.eng	passive ultrasonic irrigation	de
dc.subject.eng	conventional syringe irrigation	de
dc.subject.eng	enterococcus faecalis	de
dc.subject.eng	endodontic	de
dc.subject.eng	biofilm	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E32F-5-0
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Medizin (PPN619874732)	de
dc.subject.gokfull	Zahn-, Mund- und Kieferheilkunde - Allgemein- und Gesamtdarstellungen (PPN619876360)	de
dc.description.embargoed	2018-02-27
dc.identifier.ppn	1011551691

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Human- und Zahnmedizin [2872]

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