Mechanical and biochemical stimulation of suspended cells in a microfluidic device probed with dual optical tweezers

Rezvani Boroujeni, Samaneh

dc.contributor.advisor	Schmidt, Christoph F. Prof. Dr.
dc.contributor.author	Rezvani Boroujeni, Samaneh
dc.date.accessioned	2018-01-30T11:08:27Z
dc.date.available	2018-01-30T11:08:27Z
dc.date.issued	2018-01-30
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-002E-E33B-9
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-6701
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	530	de
dc.title	Mechanical and biochemical stimulation of suspended cells in a microfluidic device probed with dual optical tweezers	de
dc.type	doctoralThesis	de
dc.contributor.referee	Janshoff, Andreas Prof. Dr.
dc.date.examination	2017-11-17
dc.subject.gok	Physik (PPN621336750)	de
dc.description.abstracteng	Cells communicate with their environment through biochemical and mechanical interactions. They can respond to stimuli by undergoing shape- and, in some situations, volume changes. Key determinants of the mechanical response of a cell are the viscoelastic properties of the actomyosin cortex, effective surface tension, and osmotic pressure. It is challenging to measure the mechanical response of cells while changing environmental conditions. We here demonstrate the use of a novel microfluidic device with integrated hydrogel micro-windows to change solution conditions for cells suspended by optical traps. Solution conditions can be rapidly changed in this device without exposing the cells to direct fluid flow. We use biochemical inhibitors and varying osmotic conditions and investigate the time-dependent response of individual cells. Using a dual optical trap makes it possible to probe the viscoelasticity of suspended cells by active and passive microrheology and to quantify force fluctuations generated by the cells at the same time.	de
dc.contributor.coReferee	Heussinger, Claus Dr.
dc.contributor.thirdReferee	Müller, Marcus Prof. Dr.
dc.contributor.thirdReferee	Klumpp, Stefan Prof. Dr.
dc.contributor.thirdReferee	Neef, Andreas Dr.
dc.subject.eng	optical tweezers	de
dc.subject.eng	microfluidic device	de
dc.subject.eng	microrheology	de
dc.subject.eng	osmotic pressure	de
dc.subject.eng	viscoelastic properties	de
dc.subject.eng	suspended cells	de
dc.subject.eng	time-resolved response	de
dc.subject.eng	actomyosin cell cortex	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E33B-9-7
dc.affiliation.institute	Fakultät für Physik	de
dc.identifier.ppn	1011916231

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Name:: RezvaniSamaneh-Dissertation2017.pdf
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Format:: PDF
Beschreibung:: PhD Dissertation-2017

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Fakultät für Physik (inkl. GAUSS) [925]

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