Hybrids of SNARE Transmembrane Domains and Artificial Recognition Motifs as Membrane Fusion Inducing Model Peptides

Wehland, Jan-Dirk

dc.contributor.advisor	Diederichsen, Ulf Prof. Dr.
dc.contributor.author	Wehland, Jan-Dirk
dc.date.accessioned	2018-03-13T10:07:21Z
dc.date.available	2018-03-13T10:07:21Z
dc.date.issued	2018-03-13
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-002E-E384-1
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-6767
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-6767
dc.language.iso	eng	de
dc.publisher	Niedersächsische Staats- und Universitätsbibliothek Göttingen	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	540	de
dc.title	Hybrids of SNARE Transmembrane Domains and Artificial Recognition Motifs as Membrane Fusion Inducing Model Peptides	de
dc.type	doctoralThesis	de
dc.contributor.referee	Diederichsen, Ulf Prof. Dr.
dc.date.examination	2017-12-08
dc.description.abstracteng	Protein mediated fusion of membranes is a crucial biological process. For instance, SNARE (soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein receptor) proteins are involved in neuronal exocytosis. However, the knowledge about mechanistic details is still incomplete. The herein presented peptide hybrids based on the native linker and transmembrane domains of the SNARE key actors synaptobrevin 2 (Syb) and syntaxin 1A (Sx) as well as artificial recognition units based on peptide nuleic acids (PNA) or coiled-coil forming peptides (E3/K3) constitute a well controllable tool for the investigation of SNARE mediated membrane fusion. The focus is on C-terminally modified transmembrane domains which were mainly tested in FRET (Förster resonance energy transfer) related bulk leaflet mixing experiments. It was revealed that increased C-terminal negative charges hamper the fusion efficiency, whereas the removal of charges amplifies membrane fusion.	de
dc.contributor.coReferee	Jahn, Reinhard Prof. Dr.
dc.contributor.thirdReferee	Tittmann, Kai Prof. Dr.
dc.contributor.thirdReferee	Feussner, Ivo Prof. Dr.
dc.contributor.thirdReferee	Das, Shoubhik Dr.
dc.contributor.thirdReferee	Thomas, Franziska Dr.
dc.subject.eng	Membrane fusion	de
dc.subject.eng	SNARE mimetics	de
dc.subject.eng	C-terminal charge of SNARE transmembrane domains	de
dc.subject.eng	FRET based bulk leaflet mixing assays	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E384-1-5
dc.affiliation.institute	Fakultät für Chemie	de
dc.subject.gokfull	Chemie (PPN62138352X)	de
dc.identifier.ppn	1015774539

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