The Influence of Substrate Elasticity and Shear Rate on Human Blood Platelet Contraction

Hanke, Jana

Time Resolved Data Acquisition, Microfluidic Designs and Algorithms

dc.contributor.advisor	Köster, Sarah Prof. Dr.
dc.contributor.author	Hanke, Jana
dc.date.accessioned	2018-09-12T08:40:21Z
dc.date.available	2018-09-12T08:40:21Z
dc.date.issued	2018-09-12
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-002E-E4A3-4
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-7053
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	571.4	de
dc.title	The Influence of Substrate Elasticity and Shear Rate on Human Blood Platelet Contraction	de
dc.title.alternative	Time Resolved Data Acquisition, Microfluidic Designs and Algorithms	de
dc.type	doctoralThesis	de
dc.contributor.referee	Köster, Sarah Prof. Dr.
dc.date.examination	2018-04-20
dc.description.abstracteng	Human blood platelets are small, anucleate cell fragments that are important for the life-saving process of haemostasis, i.e. the process of wound healing. Upon injury, blood platelets adhere to the exposed extra-cellular matrix and further spread and contract to close the wound. This process must work reliably in a multitude of different environments, including different stiffnesses of the surrounding tissue as well as various shear rates stemming from the blood flow. Employing the technique of time-resolved Traction Force Microscopy in combination with a specifically tailored differential Particle Image Velocimetry method and a self-developed microfluidic system, it is found that platelets exhibit very dynamic as well as comparably high forces taking their small size into account. The experimental results show that platelets are not mechanosensitive within the studied stiffness or shear rate ranges. This was seen both in the spatial as well as in the temporal force development. By modelling the contractile behaviour of the cells according to the data taken on various substrate stiffnesses it was concluded that due to their small size, platelets are only mechanosenstive to substrates of at least one order of magnitude softer than used here. The only adaptation to their environment that was observed was the angle of orientation between the contraction and the flow direction. Here, the orientation changed from 45° to 90° with increasing shear rate. From the experimentally indicated correlation between the force-transmitting network and the observed spatial force patterns, using numerical simulation, the orientation of platelets under flow may be governed by the active reduction of the stress exerted on the integrins which connect the cells to the underlying substrate. In conclusion, platelets, due to their unique structure, are mechanoinsensitive to a multitude of different environments, indicating an 'all-or-nothing' response to wounds at such sites.	de
dc.contributor.coReferee	Steinem, Claudia Prof. Dr.
dc.subject.eng	human blood platelet	de
dc.subject.eng	time-resolved Traction Force Microscopy	de
dc.subject.eng	differential Particle Image Velocimetry	de
dc.subject.eng	microfluidics	de
dc.subject.eng	fluorescence microscopy	de
dc.subject.eng	biophysics	de
dc.subject.eng	single cells	de
dc.subject.eng	mechanosensitivity	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E4A3-4-3
dc.affiliation.institute	Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB)	de
dc.subject.gokfull	Biologie (PPN619462639)	de
dc.identifier.ppn	1030745137

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GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften [1115]
GGNB - Göttingen Graduate Center for Neurosciences, Biophysics and Molecular Biosciences

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