Antibakterielle Wirksamkeit schall- und ultraschallaktivierter Wurzelkanalspülungen auf einen Enterococcus faecalis-Biofilm
Comparison of the antibacterial efficacy of sonic- and two ultrasonic-activated irrigation techniques in reducing intracanal Enterococcus faecalis populations
von Felix Zimmermann
Datum der mündl. Prüfung:2018-09-18
Erschienen:2018-09-13
Betreuer:Dr. Tina Rödig
Gutachter:PD Dr. Sabine Sennhenn-Kirchner
Gutachter:Prof. Dr. Martin Oppermann
Dateien
Name:Antibakterielle Wirksamkeit schall- und ultr...pdf
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Zusammenfassung
Englisch
Objective: Investigation of the antibacterial efficacy of one sonic and two ultrasonic irrigation techniques using 1% NaOCl as an irrigant in straight human root canals infected with Enterococcus faecalis (E. faecalis). Materials and Method: Seventy extracted, single-rooted, mature, human teeth were prepared with FlexMaster NiTi-instruments, sterilized, inoculated with E. faecalis and incubated for 72 h to allow the formation of an E. faecalis-biofilm. Sixty roots were randomly divided into four experimental groups (n = 10) and infection and sterile control. The experimental groups were: syringe irrigation, sonic activation, ultrasonic activation (intermittent flush) and ultrasonic activation (continuous flush). The remaining roots were utilized for SEM analysis of the formation of E. faecalis-biofilm (n=5) and smear layer removal (n=5). Disinfection in the experimental groups was performed using 1% NaOCl within 3 minutes of sonic activation with the EndoActivator device or continuous or intermittent ultrasonic activation. After disinfection, dentine shavings were collected, diluted, plated on Columbia Blood Agar and counted after 24 h of incubation. Statistical analysis was performed using nonparametric rank-based ANOVA. Results: A significant reduction of bacterial load was achieved in all of the experimental groups compared to the infection control (p < 0,0001). There was no significant difference regarding the antibacterial efficacy between the three experimental groups (p > 0,45). Analysis of SEM images had proven a successful removal of smear layer before and the formation of a dense biofilm after incubation with E. faecalis. Conclusion: In this study, sonic and ultrasonic activation of 1% NaOCl did not increase the antimicrobial efficacy of the irrigant compared to syringe irrigation alone.
Keywords: Enterococcus faecalis; E. faecalis; irrigation; root canal; sonic; ultrasonic; EndoActivator