| dc.contributor.advisor | Janshoff, Andreas Prof. Dr. | |
| dc.contributor.author | Nehls, Stefan | |
| dc.date.accessioned | 2018-12-05T09:14:24Z | |
| dc.date.available | 2018-12-05T09:14:24Z | |
| dc.date.issued | 2018-12-05 | |
| dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-002E-E528-3 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7180 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject.ddc | 571.4 | de |
| dc.title | Cortical Tension of Cells: From Apical Membrane Patches to Patterned Cells | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Janshoff, Andreas Prof. Dr. | |
| dc.date.examination | 2018-02-13 | |
| dc.description.abstracteng | During their lifetime, cells and tissues have to deal with a variety of mechanical
challenges. Shear forces exist inside of vessels, bones have to carry the weight of
organisms and connective tissues are generally deformed on a regular basis. The
elasticity moduli of human tissues differ by orders of magnitude depending on the
tissue type, and the successful homeostasis of elasticity is required for proper function.
Cellular mechanics can be described in different ways. Here, indentation studies on
apical membranes on living MDCKII cells as well as on isolated apical membrane
sheets, originating also from Madin-Darbey Canine Kidney (MDCKII) cells, are
presented. Preparation of apical cortices is performed using porous substrates with
holes of 1.2 m diameter that are open on both sides. These studies will provide
evidence that an intact mechanical cortex can be obtained and behave mechanically
similar to living cells, as addition of crosslinking agents like glutaraldehyde will stiffen
the patches while treatment with PronaseE will soften them. These results suggest
that only a thin layer of cells determines their mechanical response to indentation.
Further indentation experiments on an epithelial cell layers show a Correlations of
surface mechanical properties of cells with their projected area. To investigate this
correlation, micropatterned substrates were created, providing small but precisely
shaped islands of extracellular matrix proteins with a non-adhesive surrounding on
standard culture dishes’ glass surfaces. Cells in different shapes and areas are used in
nanoindentation experiments, creating reliable maps of surface mechanical parameters.
The indentation data was analysed by both, a continuum model resulting in Young’s
moduli and a tension model yielding a prestress and a compressibility modulus.
Characteristic differences were found depending on the geometrical condition of the
cells and are discussed here, resembling a possible mechanism for cells to recognize
their shape. | de |
| dc.contributor.coReferee | Meinecke, Michael Prof. Dr. | |
| dc.subject.eng | Atomic Force Microscopy | de |
| dc.subject.eng | Cell Patterning | de |
| dc.subject.eng | Cell Mechanics | de |
| dc.subject.eng | Tension Model | de |
| dc.subject.eng | Cell membrane Patches | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E528-3-1 | |
| dc.affiliation.institute | Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) | de |
| dc.subject.gokfull | Biologie (PPN619462639) | de |
| dc.identifier.ppn | 1041965281 | |