Chromophore-independent roles of Drosophila opsin apoproteins and visual cycle components
von Radoslaw Katana
Datum der mündl. Prüfung:2018-11-23
Erschienen:2019-02-13
Betreuer:Prof. Dr. Martin Göpfert
Gutachter:Prof. Dr. Martin Göpfert
Gutachter:Prof. Dr. André Fiala
Gutachter:Dr. Manuela Schmidt
Gutachter:Dr. Gerd Vorbrüggen
Gutachter:Prof. Dr. Jörg Großhans
Gutachter:Dr. Jan Clemens
Dateien
Name:Radoslaw Katana thesis.pdf
Size:4.23Mb
Format:PDF
Zusammenfassung
Englisch
Visual rhodopsins consist of opsin protein moieties that are covalently bound to 11-cis-retinal, the visual chromophore that is derived from vitamin A. In Drosophila, the visual opsin genes Rh5 and Rh6 were reported to be expressed also in auditory sensory cells, contributing to mechanical amplification in hearing and sound transduction. In my thesis I report that these opsin functions are visual chromophore in-dependent. No auditory phenotypes ensued when flies were raised on vitamin-A depleted medium, and disrupting genes implicated in the de novo synthesis of 11-cis-retinal from vitamin A, i.e. ninaB and ninaD, likewise left hearing un-affected. Genes implicated in the re-cycling of 11-cis-retinal, i.e. pinta, ninaG, rdhB, and pdh, however, all turned out to be required for hearing, indicating that although the visual chromophore is dispensable for hearing, Drosophila auditory sensory cells might share the genetic pathway that, in Drosophila photoreceptors, mediates visual chromophore recycling.
Keywords: Chromophore; Opsin; Drosophila hearing