dc.contributor.advisor | Chua, John Dr. | |
dc.contributor.author | Kalantary Dehaghi, Tahere | |
dc.date.accessioned | 2019-02-25T10:23:29Z | |
dc.date.available | 2019-02-25T10:23:29Z | |
dc.date.issued | 2019-02-25 | |
dc.identifier.uri | http://hdl.handle.net/11858/00-1735-0000-002E-E5AB-C | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7310 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7310 | |
dc.identifier.uri | http://dx.doi.org/10.53846/goediss-7310 | |
dc.language.iso | eng | de |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.ddc | 570 | de |
dc.title | Study of a kinesin adaptor in axonal transport and synapse formation | de |
dc.type | doctoralThesis | de |
dc.contributor.referee | Chua, John Dr. | |
dc.date.examination | 2018-06-27 | |
dc.description.abstracteng | To form a synaptic terminal, various components have to be targeted to specific
sites in the axon. Presynaptic proteins are synthesized in the cell soma, packaged
in vesicular cargos and transported by the motor complexes traveling along microtubules. During synapse development and maturation, the intracellular transport of proteins is highly regulated by mechanisms governing the interactions and movement of motor proteins. Additional specificity and regulation is provided by interaction of motor proteins with their cargos through adaptor and scaffolding proteins. Previous studies have shown that FEZ1 acts as an adaptor for kinesin-1 and its binding to the motor is regulated by serine-58 phosphorylation. FEZ1/kinesin-1 complex partakes in presynapse formation by transporting syntaxin and Munc18 in the axon. Identification of numerous synaptic vesicle and active zone proteins in the FEZ1/kinesin-1 immunoisolated vesicles suggested the association and transport of these components with this complex. Although functional studies in Caenorhabditis elegans showed synaptic disorganization, the relevance of mammalian FEZ1 in neurons have not yet been investigated.
In this study, the mobility of FEZ1 and its phosphomutants were characterized in
mammalian neurons. Furthermore, the role of FEZ1-mediated transport in formation
of presynaptic and postsynaptic specializations was investigated by ablating the
expression of FEZ1 using CRISPR/cas9 knockdown system. The data in this study
show that mobility of neuronal FEZ1 is a result of precise modulation of its binding
to kinesin-1 by serine-58 phosphorylation. This study also provides evidence for
FEZ1 role in transport of active zone components and synaptic vesicle precursors,
albeit in distinct mechanisms. Additionally, FEZ1 impacts neuronal development
by regulating dendritic branching in young neurons, and dendritic spine formation
during later stages. | de |
dc.contributor.coReferee | Klopfenstein, Dieter Dr. | |
dc.contributor.thirdReferee | Jakobs, Stefan Prof. Dr. | |
dc.subject.eng | Axonal transport | de |
dc.subject.eng | FEZ1 | de |
dc.subject.eng | kinesin | de |
dc.subject.eng | motor protein | de |
dc.subject.eng | synaptogenesis | de |
dc.identifier.urn | urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E5AB-C-5 | |
dc.affiliation.institute | Göttinger Graduiertenschule für Neurowissenschaften, Biophysik und molekulare Biowissenschaften (GGNB) | de |
dc.subject.gokfull | Biologie (PPN619462639) | de |
dc.identifier.ppn | 1066542600 | |