Die Rolle der Proteindisulfidisomerase ERp57 in der Chemoresistenz des Nierenzellkarzinoms

Katzendorn, Olga

dc.contributor.advisor	Dihazi, Hassan Prof. Dr.
dc.contributor.author	Katzendorn, Olga
dc.date.accessioned	2019-03-14T13:29:35Z
dc.date.available	2019-04-04T22:50:04Z
dc.date.issued	2019-03-14
dc.identifier.uri	http://hdl.handle.net/11858/00-1735-0000-002E-E5CB-6
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-7342
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-7342
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.ddc	610	de
dc.title	Die Rolle der Proteindisulfidisomerase ERp57 in der Chemoresistenz des Nierenzellkarzinoms	de
dc.type	doctoralThesis	de
dc.title.translated	The impact of the proteine disulfite isomerase ERp57 in chemoresistance of renal cell carcinoma	de
dc.contributor.referee	Dihazi, Hassan Prof. Dr.
dc.date.examination	2019-03-21
dc.description.abstracteng	It is well-known that renal cell carcinoma is resistant to conventional chemotherapeutic drugs. In the past serious investigation has been made to understand the underlying pathomechanisms. To analyse the mechanism of resistance to a classical chemotherapeutic drug in renal cell carcinoma cell lines, two commercial renal carcinoma cell lines, A498 and Caki-2, were treated with cisplatin and differentially expressed proteins were identified. The Caki-2 cell line was sensitive to treatment with Cisplatin, whereas the A489 cell line was not. Proteome changes after treatment with cisplatin have been shown in both cell lines. In former studies two statistically significant upregulated proteins in A498 cells after treatment with cisplatin were associated with chemoresistance. Further investigation will be necessary to elucidate whether these proteins are key players in resistance of renal cell carcinoma. Furthermore we showed upregulation of proteins being part of the „unfolded protein response“ (UPR). The UPR is activated in order to decrease ER-stress which is provoked by the accumulation of nascent proteins in the ER under stressful conditions. ER stress as well as proteins connected to the UPR, like the chaperons ERp57 or GRP78, have been linked to chemoresistance and cancer. ERp57 is an ER stress protein which displayed upregulation in A498 cells and downregulation in Caki-2 cells after treatment with cisplatin. To investigate its role in chemoresistance, we downregulated and upregulated its expression in both cell lines. We could not prove a key role in chemoresistance, whereas it has to be assumed that this protein is a crucial player in the regulation of cellular homeostasis. Further investigation will be needed to prove this assumption. Moreover, we could not confirm a positive effect of ERp57 on cell proliferation as described earlier.	de
dc.contributor.coReferee	Urlaub, Henning Prof. Dr.
dc.subject.eng	chemoresistance	de
dc.subject.eng	erp57	de
dc.subject.eng	renal cell carcinoma	de
dc.subject.eng	ER-stress	de
dc.identifier.urn	urn:nbn:de:gbv:7-11858/00-1735-0000-002E-E5CB-6-6
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Bibliotheken {Medizin} (PPN619874953)	de
dc.description.embargoed	2019-04-04
dc.identifier.ppn	1666649201

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Human- und Zahnmedizin [2871]

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