The role of mammalian TRC40 in membrane-protein targeting and chaperoning

von Francisco Javier Coy Vergara
Dissertation
Datum der mündl. Prüfung:2018-06-04
Erschienen:2019-05-24
Betreuer:Prof. Dr. Blanche Schwappach
Gutachter:Prof. Dr. Blanche Schwappach
Gutachter:Prof. Dr. Michael Kessel
Gutachter:Dr. Nuno Raimundo
Gutachter:Prof. Dr. Michael Meinecke
Gutachter:Dr. Hans Dieter Schmitt
Gutachter:Prof. Dr. Ralph Kehlenbach
Zum Verlinken/Zitieren: http://dx.doi.org/10.53846/goediss-7455

 

 

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Description:The role of mammalian TRC40 in membrane-protein targeting and chaperoning

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Zusammenfassung

Englisch

Tail-anchored (TA) proteins are distinguished from other membrane proteins due to their particular topology. The best-characterized pathway for the targeting of TA-proteins is the GET pathway in yeast or the TRC pathway in mammals. Recently, several studies have reported that more than one post-translational pathway operate during targeting of TA-proteins to the ER-membrane such as the EMC pathway, Hsp40/Hsc70, the SND pathway and the PEX pathway. TRC40 is the cytoplasmic effector of the TRC pathway. This study aims to investigate the reliance of TA-proteins on the TRC pathway at the steady-state in vivo in mammalian cells. Moreover, the role of several functional domains of TRC40 during TA-proteins targeting to ER membrane and chaperoning in vivo is addressed in this study. Furthermore, this study wants to explore the potential alternative role of TRC40 as redox-regulated chaperone.
Keywords: TRC40; TRC pathway; Client spectrum; Chaperone; Tail-anchored proteins; TA-proteins; Trap
 
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