Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Köster, Sarah Prof. Dr."
Now showing items 1-20 of 28
-
Actin turnover regulates mechanical properties of oligodendrocytes and myelin formation
(2015-10-02)The myelin sheath is a specialized membranous structure that facilitates rapid signal conduction along axonal segments. During central nervous system development, it is formed by oligodendrocytes that extend motile and ... -
Active and Passive Microrheology of F-Actin Membrane Composites
(2018-07-19)The complex mechanical properties of a living cell are not only a function of its structural components but also of the organizational super-structure and the dynamic interconnection of filaments, proteins and the plasma ... -
Automated STED microscopy for cell-biological high-throughput assays
(2022-11-28)In the past decade, super-resolution fluorescence microscopy has revolutionized biological research by enabling the study of biological functions down to the molecular scale. Despite profound technological developments, ... -
Blood Platelet Behavior on Structured Substrates
(2015-11-02)Blood clotting is the immediate answer of the body to injuries. In this process, blood platelets adhere to the injured site, spread over the wound and form a blood clot, in which single platelets are connected by fibrin ... -
CFM: Confinement Force Microscopy-a dynamic, precise and stable microconfiner for traction force microscopy to study confined cell behaviour
(2024-01-11)Physical properties of the 3D microenvironment and the residing tissue affect cell behavior and function. 3D environment represents major physical obstacles for cell migration, such as spatial confinement, which could ... -
Collective Mechanical Behavior of Epithelial Cells - The Impact of Micro-Wounding
(2019-06-24)For epithelia, which are lining organs or blood vessels, the mechanical integrity of a whole cell sheet is indispensable. The question arises, how neighboring cells mechanically work together to build a functioning cell ... -
Dynamics and mechanics of adherent cells in the context of environmental cues
(2015-03-25)Cellular behavior is influenced by many biochemical but also physical factors in the direct cellular environment. Thereby, cells not only react to external cues, the interaction between cells and their environment is also ... -
Dynamics and self-organization of membrane-bound minimal actin and actomyosin cortices based on ezrin-PtdIns[4,5]P2 linkage
(2022-11-22)The cellular cortex is a thin actin layer attached to the cytoplasmatic plasma membrane leaflet of mammalian cells. Through a complex interaction with hundreds of actin binding proteins (ABPs), controlling e.g. the cortex ... -
Dynamics of Surfactants at Soft Interfaces using Droplet-Based Microfluidics
(2016-01-26)In this work, I studied the impact of surfactants on interfacial phenomena out of equilibrium. I investigated three different effects, (1) the stabilisation of interfaces upon surfactant adsorption (adsorption kinetics), ... -
Fluorescence correlation spectroscopy for studying intermediate filament assembly
(2017-08-10)Intermediate filaments play a central role in the cytoskeleton of eukaryotic cells. Together with microtubules and actin filaments they determine the mechanical properties of cells. Microtubules are also the guiding tracks ... -
Interactions between cytoskeletal filaments in cells
(2024-10-04)Essential functions of eukaryotic cells such as cell division, force generation and the ability to mechanically adapt to the outer environment depend on the cytoskeleton, which is an intricate network containing three ... -
Intermediate filament mechanics across scales
(2022-02-24)The mechanical properties of cells are largely determined by the cytoskeleton. The cytoskeleton is an intricate and complex structure formed by protein filaments, motor proteins, and crosslinkers. The three main types of ... -
Keratin Networks in Live Cells
(2014-09-26)Intermediate filaments are a major component of the eukaryotic cytoskeleton along with microtubules and microfilaments and play a key role in cell mechanics. One representative of this class of proteins, which exemplifies ... -
Mechanics and Motility of Epithelial Cells: From Single Cell Behavior to Collective Migration
(2023-01-13)Mechanical behavior of cells plays a crucial role in a plethora of biological processes. Despite its importance, this aspect of life science has only more recently gained increasing interest. Yet, without mechanical force ... -
Mechanics of suspended cells probed by dual optical traps in a confocal microscope
(2015-12-02)Cells are sensitive to mechanical cues from their environment and at the same time generate and transmit forces to their surroundings. In this thesis, we quantitatively measured forces and elastic spring constants of ... -
Membrane tension homeostasis of mammalian cells
(2017-04-12)Although a cell is the smallest unit that is viable on its own, its construction and processes are of great complexity. Many functions, which keep a cell alive, are only poorly understood up to now. Plasma membrane tension ... -
Microfluidic cryofixation for time-correlated live-imaging cryo-fluorescence microscopy and electron microscopy of Caenorhabditis elegans
(2018-11-01)Light and electron microscopy are complementary methods to study biological systems at the cellular and sub-cellular scale. Light microscopy is compatible with live cells, allowing features of interest to be selectively ... -
Modelling the Dynamics of Actomyosin Networks
(2022-11-04)In this dissertation, a new framework called bead-state model (BSM) for computer simulations of actin networks is presented, and its scientific validity and its usability and flexibility is demonstrated in three ... -
Multicolor 3D MINFLUX nanoscopy for biological imaging
(2020-11-12)The resolution of conventional optical fluorescence far-field microscopes is limited by the diffraction of light. This implies that only features in a distance of about half of the wavelength can be discerned. In the last ... -
Quantifying the diffusion of membrane proteins and peptides
(2013-08-15)Lipid and protein diffusion in membranes is a fundamental requirement for many signaling processes in biological cells. Therefore, measuring protein and lipid mobility in lipid bilayers with high accuracy is essential for ...