Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Jahn, Reinhard Prof. Dr."
Now showing items 21-40 of 47
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Synthesis and Analysis of Modified SNARE Proteins with Respect to Assembly and Disassembly of the SNARE Complex
(2017-01-12)In this thesis, the first aim was to synthesise an inhibitor for the alpha-SNAP/NSF-mediated disassembly reaction of the SNARE complex. Based on identified interaction sites between alpha-SNAP and Synaptobrevin-2, suitable ... -
The role of the mammalian GET pathway in the mouse liver
(2016-11-29)The correct insertion of membrane proteins is essential for the normal functioning of a eukaryotic cell, and different classes of membrane proteins employ different mechanisms of insertion into their target membrane. One ... -
Non-Canonical Amino Acids as Minimal Tags for Investigating Protein Organization and Turnover
(2016-01-08)The burgeoning field of genetic code expansion provides new tools for specifically labeling proteins for a variety of applications. Clickable non-canonical amino acids (ncAA) have been refined for almost-quantitative and ... -
Development of new methods in fluorescence microscopy
(2015-12-02)In recent years, advancements in single-molecule imaging techniques have enabled scientists to study in great detail the cells and relevant physiological processes, including neurons and the communication between them. ... -
Molecular Mechanisms of Astrocyte Vesicle Fusion at Synaptic Interfaces
(2015-12-01)Astrocytes are key to neuronal trophic support, development, and synaptic signalling and plasticity. Astrocytes can detect synaptic events at tripartite synapses –a major site of bidirectional communication between neurones ... -
Role of Munc13 Isoforms in Regulating Large Dense Core Vesicle Exocytosis in Chromaffin Cells
(2015-11-03)The process of vesicular exocytosis is a fast, tightly regulated and Ca2+-triggered event mediated by SNARE complex formation. Although it is known that factors like Munc18, Munc13 and CAPS are important in setting up ... -
In vitro investigation of trans SNARE complexes arrested between artificial membranes
(2015-09-23)The key components of the machinery that catalyzes membrane fusion include membrane-associated small proteins that are termed as soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). Calcium-triggered ... -
Molecular and Morphological Correlates of Synaptic Vesicle Priming
(2014-10-27)Excitation-secretion coupling at nerve cell synapses is a sub-millisecond process that entails the transduction of an electrical stimulus into synaptic vesicle fusion. Before fusion, synaptic vesicles are physically docked ... -
Role of DLG-MAGUKs in surface NMDAR localization and its patho-physiological functions
(2014-10-16)The post-synaptic side of an excitatory synapse is characterized by an electron-dense, highly dynamic architecture of proteins, called the post-synaptic density or PSD. PSDs comprises of a diverse group of proteins, from ... -
Molecular mechanisms of collybistin-dependent gephyrin clustering at inhibitory synapses
(2014-07-03)Information processing and transmission in neuronal networks in the mammalian brain occurs through intercellular communication between neurons at synapses. Inhibitory synapses play a key role, for example to maintain network ... -
Spatial-temporal actin dynamics during synaptic plasticity of single dendritic spine investigated by two- photon fluorescence correlation spectroscopy
(2013-12-19)In the content of my thesis, the construction and calibration of a setup that combined conventional wide field fluorescence microscopy (FM) and two-photon fluorescence correlation spectroscopy (2P-FCS) was first described. ... -
Mechanism of regulation of spliceosome activation by Brr2 and Prp8 and links to retinal disease
(2013-11-12)Splicing is a crucial post-transcriptional processing event that entails the removal of non-coding intervening sequences (introns) from eukaryotic pre-mRNA and the ligation of the coding sequences (exons). It is carried ... -
Cellular function and toxicity of the Parkinson’s disease-related genes α-synuclein and catp-6 in C. elegans
(2013-04-09)Parkinson’s disease (PD) is the second most common neurodegenerative disease in industrialized countries. However, the molecular mechanisms leading to neuronal death in PD still remain unclear. About 5-10% of PD patients ... -
Development of Fluorescence Activated Synaptosome Sorting (FASS) and analysis of VGLUT1 synapses from mouse brain
(2012-10-05)Signal propagation between neurons in the brain is mediated by chemical synapses. Depending on the neurotransmitters and their receptors, synapses can transmit excitatory, inhibitory, or modulatory signals. A detailed ... -
Dense-core vesicle maturation at the Golgi-endosomal interface in Caenorhabditis elegans
(2012-06-08)Fast synaptic transmission is mediated by the triggered release of neurotransmitters from synaptic vesicles (SVs). To regulate synaptic transmission and neuronal activity, neurons also release neuropeptides and hormones ... -
The identification and characterization of Mio10 and MINOS1 as novel regulators of mitochondrial inner membrane organization
(2012-06-07)Various mitochondrial inner membrane proteins contribute to the structure of cristae membranes and to the overall dynamic morphology of the inner membrane. These proteins include the F1FOATPase dimers, which contribute to ... -
Reconstituted SNARE-mediated fusion: towards a mechanistic understanding
(2012-03-20)A novel and well-characterized model system has been developed and used in order to extract mechanistic insights on the SNARE-mediated fusion process. The reconstitution of SNAREs onto large ... -
Molecular profiling of presynaptic docking sites
(2011-10-20)In presynaptic nerve endings, exocytosis of synaptic vesicles is restricted to specialized areas of the plasma membrane, called active zones, that are distinguished by electron-dense material. Here synaptic vesicles ... -
Myelin Membrane Growth and Organization in a Cellular Model System (EN)
(2011-01-19)This thesis addresses the two fundamental questions of myelin biogenesis: how the myelin membrane is extended and how it acquires its specific lateral organization. Myelin is a multilayered ... -
Investigation of Endosomal Recycling of Synaptic Vesicles
(2010-11-05)During communication at chemical synapses, small neurotransmitter-filled synaptic vesicles, housed in the pre-synaptic nerve terminal (bouton), fuse with the plasma membrane (exocytosis) ...