Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Tittmann, Kai Prof. Dr."
Now showing items 21-40 of 56
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Monitoring dynamics of protein nascent chain on the ribosome using PET-FCS
(2020-06-24)The ribosome synthesizes proteins according to the sequence of the messenger RNA (mRNA) by progressively adding amino acids to the C-terminus of the nascent peptide. For a protein to become fully functional, it has to fold ... -
New sample preparation techniques of macromolecular complexes for high resolution structure determination using cryo-EM
(2020-04-07)Fatty acids are one of the most abundant lipids in the cell. Cells use them to build biological membranes, as energy reserves and as signaling molecules. Fatty acids are synthesized by a specialized protein machinery called ... -
Reaction mechanism of hOMPD and CaAAD at atomic resolution
(2019-12-12)Decarboxylation reactions are fundamental processes in the chemistry of the animated world. Thus, the evolution of decarboxylases brought up a stunning array of diverse enzymes utilizing a variety of different mechanisms ... -
Interplay of the COP9 signalosome deneddylase and the UspA deubiquitinase to coordinate fungal development and secondary metabolism
(2019-05-29)Protein half-life is controlled through the ubiquitin-proteasome system (UPS). Proteins are labeled with ubiquitin chains by E3 cullin RING ligases (CRLs) and then degraded by the 26S proteasome. The COP9 signalosome (CSN) ... -
Structure-based mechanistic analysis of the proteasome
(2019-05-02)The ubiquitin proteasome pathway is one of the major protein degradation pathways in the cell. This pathway is essential for protein quality control and the regulation of the cell cycle. Proteins subjected to the ubiquitin ... -
Computational methods for the structure determination of highly dynamic molecular machines by cryo-EM
(2019-02-20)In the last couple of years, electron cryomicroscopy (cryo-EM) has gained of rising importance in the field of structural biology and biophysics. Not only that the routinely achievable resolution of the method has ... -
Recognition Elements for Elongation Factor P on the Ribosome
(2019-01-25)Translation elongation is a multi-step process orchestrated by elongation factors. Elongation factors G and Tu are required for each round of translation elongation, whereas elongation factor P is only required to assist ... -
Mechanistic Characterization of Transaldolase from <i>Thermoplasma Acidophilum</i> and Preliminary Analysis of the QncN/L-M Protein System from <i>Streptomyces Melanovinaceus</i>
(2018-02-13)The results introduced in the present work are mainly based on the pre-steady state and steady-state analysis supported by structural information from crystallographic studies. Different variants of TacTAL were generated ... -
Kinetics of subunit rotation of the ribosome during tRNA-mRNA translocation
(2017-07-25)Ribosome dynamics play an essential role in orchestrating all stages of protein synthesis. Recent biochemical, structural and computational studies have shown large-scale conformational changes of the ribosome, its tRNA ... -
Single Molecule Cryo-Fluorescence Microscopy
(2016-11-07)This work presents a newly developed cryo-fluorescence microscopy system and several of its applications. The thesis is divided into three parts. Part I introduces the design of the cryo-fluorescence microscope, and ... -
Structure and Mechanism of the Flavoenzyme Lipoamide Dehydrogenase from Escherichia coli
(2016-08-29)The flavoenzyme lipoamide dehydrogenase (E3) is an ubiquitously distributed enzyme which is part of several multienzyme complexes involved in cellular carbon metabolism thus contributing to cellular homeostasis. It catalyzes ... -
Computational study of the molecular details of ion permeation across the formate-nitrite transporters
(2016-07-12)The selective flux of molecules across biological membranes is essential for the normal function of any cell, and is governed by diverse membrane transport proteins. Historically, the focus in the membrane transport field ... -
Structural Survey on Cohesin and Viomycin Inhibited 70S Ribosome by Single Particle Electron Microscopy
(2016-04-21)Life can be understood as the systematic collaborative action of several entities on molecular level reducing locally the entropy at the cost of the exterior environment. These entities are in great part macromolecular ... -
Functional dynamics of the anti-HIV lectin OAA and NMR methodology for the study of protein dynamics
(2016-01-26)Specific recognition between biomolecules is at the basis of all biological processes, and a mechanistic description of molecular recognition is crucial for a detailed understanding of these processes. Two limiting models ... -
NMR Spectroscopy of the Tau-Microtubule Interaction
(2015-12-10)Tau is an intrinsically disordered, microtubule associated protein and is responsible for the promotion of microtubule (MT) formation and stabilization. The human Tau protein has six different isoforms, which are expressed ... -
Characterizing the Structure and Mechanics of 2D Clathrin Lattices with Atomic Force Microscopy
(2015-10-30)Clathrin is a self-assembling protein involved in intracellular trafficking. Since its discovery in the early 1960s, much has been learned about its structure, assembly, and regulation mechanisms. The three legged monomers, ... -
Biochemical Characterization of Human Guanylate Kinase and Mitochondrial Thymidine Kinase: Essential Enzymes for the Metabolic Activation of Nucleoside Analog Prodrugs
(2015-07-28)Deoxyribonucleoside and nucleotide kinases are key enzymes that catalyze the critical phosphorylation steps in the conversion of antiviral and anticancer nucleoside analogs to their corresponding cytotoxic nucleoside ... -
Interaction of the SecYEG translocon with the SRP receptor and the ribosome
(2015-05-08)This work aimed at studying the interaction between SecYEG and FtsY and before and after the translating ribosome has been transferred to the SecYEG translocon. In this study we have used monomers of the SecYEG complex ... -
Crystallization and biophysical characterization of spliceosomal protein complexes
(2015-02-20)mRNA splicing is a highly dynamic process by which non-coding sequences are removed from a pre-mature mRNA. It is catalyzed by the spliceosome, a huge and highly dynamic ribonucleoprotein complex consisting of up to five ... -
Conformational Dynamics of large protein Complexes
(2015-02-18)Life on earth is only possible through the enormous capabilities of proteins and their assemblies. Which function a certain protein fulfills is encoded in its amino acid sequence, which gives rise to a defined structure. ...