Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Title
Now showing items 102-121 of 1236
-
Bildung und Homöostase von c-di-AMP in Bacillus subtilis
(2014-07-14)Signal transduction describes the ability of cells to sense environmental stimuli and transfer this information in order to react appropriately to these stimuli. In this process, signaling nucleotides are often important. ... -
Biochemical analysis of CYP74-enzymes in Physcomitrella patens
(2014-03-18)In this study recombinant CYP74-enzymes AOS1, AOS2 and HPL from the moss P. patens, DES from S. tuberosum and EAS from the lancelet B. floridae were expressed in E. coli and purified for further analysis. PpAOS1 and BfEAS ... -
Biochemical and functional differences of chromatin assembled replication-coupled or independent in Xenopus laevis egg extracts
(2012-04-12)Two main chromatin assembly pathways ensure the proper transmission of chromatin organization and chromatin-based information throughout the cell cycle. A replication-dependent (RD) pathway that couples chromatin assembly to ... -
Biochemical Characterization and Engineering of L-asparaginases for Amino Acid Depletion Therapy of Acute Lymphoblastic Leukemia
(2014-06-25)The goal of the present work was to form a basis for the development of improved protein therapeutics against acute lymphoblastic leukemia (ALL). Bacterial L-asparaginases, in combination with other chemotherapeutics, are ... -
Biochemical Characterization of Human Guanylate Kinase and Mitochondrial Thymidine Kinase: Essential Enzymes for the Metabolic Activation of Nucleoside Analog Prodrugs
(2015-07-28)Deoxyribonucleoside and nucleotide kinases are key enzymes that catalyze the critical phosphorylation steps in the conversion of antiviral and anticancer nucleoside analogs to their corresponding cytotoxic nucleoside ... -
Biochemical Characterization of Otoferlin, the Ca2+ sensor for exocytosis at inner hair cell synapses
(2025-03-27)Transmission of sound from the ear to the brain requires high temporal and spatial fidelity. Inner hair cells (IHC) of the inner ear are the primary sensory organ of hearing, forming the first synapse of the auditory ... -
Biochemical characterization of presynaptic membrane protein complexes
(2017-09-13)At chemical synapses, synaptic vesicles, membrane-enclosed entities filled with neurotransmitters, undergo exocytosis at restricted areas of the presynaptic membrane called active zones (AZ). The core AZ proteins form a ... -
Biochemical characterization of the Nup62⋅58⋅54 nucleoporin complex and mutational analysis of the exportin CRM1
(2014-10-15)Nuclear pore complexes (NPCs) are giant molecular machines embedded in the double membrane that surrounds the eukaryotic nucleus. Deciphering the molecular structure of the NPCs is critical to our understanding of both ... -
Biochemical reconstitution of human autophagy initiation
(2023-05-04)Autophagy is a tightly regulated process that eukaryotic cells use as a major survival mechanism to reallocate nutrients to essential processes in adverse conditions such as nutrient or energy deprivation. Autophagy is ... -
Biogenesis and maintenance of cytoplasmic domains in myelin of the central nervous system
(2017-02-27)Myelin is a multi-lamellar membrane structure, produced by oligodendrocytes which are special glial cells, that myelinate axons in the central nervous system (CNS) (Aggarwal, Yurlova, & Simons, 2011; Vassall, Bamm, & Harauz, ... -
Biogenesis of Lipid Bodies in Lobosphaera incisa
(2017-02-28)Microalgae hold great promise with regards to the production of valuable products such as PUFAs and biofuel. They are a highly interesting group of organisms for investigating lipid metabolism and while some insight has ... -
Bioinformatics analysis of multi-omics data elucidates the primary function of Oct4 in gene regulation and pluripotency maintenance
(2022-03-14)The transcription factor (TF) Oct4 is essential for maintaining pluripotency in vitro as well as in vivo. The crucial function of Oct4 in control of pluripotency has been extensively studied during reprogramming of somatic ... -
Bioinformatics analysis of multi-omics data elucidates U2 snRNP function in transcription
(2021-08-16)Transcription by RNA polymerase II (Pol II) is an important step in cell function and regulation. Pol II transcription has been shown to be coupled to pre-mRNA splicing, but the underlying mechanisms remain poorly ... -
Biophysical and Crystallographic Characterization of Spliceosomal DExD/H-box ATPases
(2020-04-23)DEAH-box ATPases play a vital role in the activation, catalytic and disassembly steps of the spliceosome. They are key for the orchestration of conformational and compositional rearrangements during the splicing cycle. It ... -
Biophysical and molecular mechanisms of mean and variance adaptation in the Drosophila ear
(2023-05-16)Adaptation is an important mechanism found across sensory systems and species. This important ability of sensory systems expands our range of perception. Giving us the ability to perceive a quiet whisper as well as a loud ... -
Biophysical modeling of information processing in the <i>Drosophila</i> olfactory system
(2014-04-24)Fruit flies (Drosophila melanogaster) rely on their olfactory system to process environmental information. Although extensive studies have helped neurobiologists to understand the basic molecular and cellular mechanisms ... -
Blood Platelet Behavior on Structured Substrates
(2015-11-02)Blood clotting is the immediate answer of the body to injuries. In this process, blood platelets adhere to the injured site, spread over the wound and form a blood clot, in which single platelets are connected by fibrin ... -
Bone Morphogenetic Protein signaling in the planarian flatworm Schmidtea mediterranea – evolutionary, cellular, and spatial patterns
(2025-03-13)In early development, morphogen gradients orchestrate the establishment of body axes. Perturbations of such a fundamental process are often damaging to embryonal development, making it difficult to study the role of body ... -
Brain-wide connectivity of molecularly defined GABAergic neurons in mouse barrel cortex visualized with optimized rabies virus tracing
(2020-03-16)Cortical GABAergic neurons are indispensable in controlling the activity of cortical networks. Parvalbu- min (PV), somatostatin (SST) and vasoactive intestinal polypeptide (VIP) expressing neurons are three main classes ... -
Branching control mechanisms in the model tree Populus: analyzing the role of strigolactones and BRANCHED1
(2016-05-13)Plants exhibit a large degree of phenotypic plasticity. Modifications of their genetically pre-defined body plan allow them to flexibly react to a wide range of environmental conditions. This includes changes in plant ...