Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Title
Now showing items 21-40 of 1164
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A protein in search of a function: The c-di-AMP-binding protein DarA of Bacillus subtilis
(2019-07-26)Adaptation to changing environmental conditions is crucial for any organism to thrive in nature. Bacteria like the Gram-positive model organism Bacillus subtilis have evolved so-called second messengers to facilitate ... -
A quantitative analysis of an average cultured neuron
(2020-04-02)Neurons are the functional unit of the brain. They have been extensively studied, from a molecular perspective up to brain wide levels. Nonetheless, we are still missing a quantitative description of the neuronal components. ... -
A quantitative analysis of the molecular organization of dendritic spines from hippocampal neurons
(2019-08-20)Dendritic spines are the major excitatory postsynaptic compartment in the brain. Despite its essential function in synaptic transmission and plasticity, we are still lacking a comprehensive and quantitative understanding ... -
A Quantitative Mass Spectrometric Analysis of Signalling Proteins in Human Sperm Cells
(2024-05-17)Fertilization of an oocyte by a sperm cell is a complex multi-stage process. First, a sperm cell must locate and reach the oocyte; second, it must undergo capacitation, the acrosome reaction, and hyperactivation to penetrate ... -
A regulatory network of Mdm2 and members of the Polycomb Group (PcG) family
(2016-01-22)The E3 ubiquitin ligase MDM2 is the most well-known physiological antagonist of the tumor suppressor P53. P53 induces cell cycle arrest or apoptosis in the case of DNA damage, whereas MDM2 targets it for proteasomal ... -
A Role For Microtubule Dynamics For The Induction Of Chromosomal Instability And Cell Migration And Invasion In Human Cancer Cells
(2017-06-21)Aneuploidy and increased cell migration and invasion are hallmarks of aggressive human cancers. Aneuploidy derives from an increased rate of perpetual chromosome missegregation during mitosis, referred to as chromosomal ... -
A scanning ion conductance microscopy assay to investigate interactions between cell penetrating peptides and pore-suspending membranes
(2014-10-10)Scanning ion conductance microscopy (SICM) provides a technique for the investigation of surface topography as well as the local ion conductance of a surface without any mechanical contact between probe and sample. This ... -
A screen for proteins involved in parasitism from Meloidogyne hapla identifies an amphid localized transthyretin-like protein as a potential effector
(2015-10-23)Root-knot nematodes (Meloidogyne sp.) present a constant threat to agriculture worldwide. When present in the field, they can infect roots and cause a distorted root structure. This strongly reduces crop production and ... -
A Single Vesicle Assay to Study the Electrochemical Gradient Regulation in Glutamatergic and GABAergic Synaptic Vesicles
(2016-02-24)During neuronal activity, synapses sustain neurotransmission by a high fidelity multi-step process called synaptic vesicle (SV) recycling. This process involves endocytosis, neurotransmitter loading and exocytosis of SVs ... -
A State Space Odyssey — The Multiplex Dynamics of Cardiac Arrhythmias
(2018-10-18)With three million people worldwide (three hundred thousand people in the United States alone) experiencing sudden cardiac arrest per year, it is one of the most common causes of death in developed countries. Ventricular ... -
A theory of inference and learning in cortex with spiking neurons and dendritic error computation
(2023-11-03)How the cortex performs its intricate computations, and how it adapts to the world around it, is one of the central mysteries in neuroscience. It is a longstanding belief that one of the main aims of the brain, and especially ... -
A twist in NMR relaxation experiments: Application to the study of protein motions
(2019-09-27)Understanding biological processes requires knowledge about the structure as well as dynamics of the involved molecules. Proteins are complex structures, which experience time dependent changes due to atomic motions. The ... -
AAV-based gene therapy for axonal regeneration in a rat model of rubrospinal tract lesion
(2015-04-29)Spinal cord injury (SCI) is a devastating and largely irreversible clinical condition, which can lead to permanent sensory and motor functional disabilities. Worldwide, an estimated 2.5 million people suffer from SCI and ... -
Aberration correction in STED microscopy
(2019-11-21)In the last decade, superresolution microscopy techniques became a valuable tool to study sub-cellular structures with a resolution below the diffraction limit. The use of superresolution for brain research requires to ... -
Absolute and relative quantification of proteins in large protein-RNA assemblies by mass spectrometry
(2010-09-07)The majority of cellular processes is driven by protein complexes. These emerge as multimeric protein assemblies or are complexed with ligands such as RNA or DNA. To understand the details ... -
Absolute quantification of peptide products in in vitro digestions: A computational approach using Bayesian inference
(2023-06-29)Insight into enzyme specificities and dynamics is central to understanding biochemical processes. The peptide products generated by purified proteases in in vitro digestions are often identified by mass spectrometry ... -
Accessing the kinetics of the supra-tauc range via relaxation dispersion NMR spectroscopy
(2013-10-30)Nuclear Magnetic Resonance spectroscopy (NMR) is capable of attaining dynamic information of motions over a broad range of time scales under physiological relevant conditions while maintaining atomic resolution. Of the ... -
Acetyltransferase TIP60/KAT5 regulates the Drosophila Circadian Clock
(2016-10-14)Endogenous clocks control behavioral and physiological processes in a rhythmic fashion exhibiting periodicity of ~24 h. Drosophila exhibits a robust rhythm in activity/rest patterns which is controlled by circadian pacemaker ... -
Actin promotes chromosome aggregation and accurate chromosome segregation in mammalian oocytes
(2022-09-15)Accurate chromosome segregation during meiosis is essential for the formation of a new life. During meiosis, spindle microtubules bind to the chromosomes’ kinetochores and arms in order to separate the genetic material. ... -
Actin turnover regulates mechanical properties of oligodendrocytes and myelin formation
(2015-10-02)The myelin sheath is a specialized membranous structure that facilitates rapid signal conduction along axonal segments. During central nervous system development, it is formed by oligodendrocytes that extend motile and ...