Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Title
Now showing items 716-735 of 1214
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New Algorithms for Macromolecular Structure Determination
(2009-10-28)Detailed three dimensional information of macromolecules is often crucial to the study of biological systems. Structural data have for example been used to elucidate the basis of diseases ... -
NEW COMPUTATIONAL METHODS FOR 3D STRUCTURE DETERMINATION OF MACROMOLECULAR COMPLEXES BY SINGLE PARTICLE CRYO-ELECTRON MICROSCOPY
(2009-04-22)In cryo-electron microscopy (cryo-EM) single-particle reconstruction, projection images of many first order structural identical copies of the specimen of interest are combined to recover ... -
New Computational Tools for Sample Purification and Early-Stage Data Processing in High-Resolution Cryo-Electron Microscopy
(2019-01-07)Large macromolecular protein complexes play an important role in the quest of un- derstanding life at a molecular level. For this endeavor, the highly-resolved 3D structure of a large complex and its changes, which are ... -
New genetic mouse models for neurodegenerative diseases: Contribution of noradrenaline to Alzheimer’s disease pathogenesis and creation of a new genetic model for Parkinson’s disease.
(2011-12-15)Neurodegenerative diseases are among the most devastating diseases for which there are no cure to date. The greatest risk factor is advancing age. A common feature of these disorders is the toxic aggregation of a peptide ... -
New redox-switches in proteins
(2024-04-26)Regulation of cellular pathways in response to the redox state of a cell is an important regulatory process. A well-established paradigm of redox-sensing is disulfide formation between two cysteine residues and cysteine ... -
New sample preparation techniques of macromolecular complexes for high resolution structure determination using cryo-EM
(2020-04-07)Fatty acids are one of the most abundant lipids in the cell. Cells use them to build biological membranes, as energy reserves and as signaling molecules. Fatty acids are synthesized by a specialized protein machinery called ... -
NFATc1 driven ER-stress responses in the progression of non-alcoholic fatty liver disease
(2021-06-30)Non-alcoholic fatty liver disease (NAFLD) mirrors the hepatic manifestation of the metabolic syndrome and is defined by progressive fat accumulation in hepatocytes (steatosis). Uncomplicated NAFLD may progress to a severe ... -
NMR Spectroscopy of the Tau-Microtubule Interaction
(2015-12-10)Tau is an intrinsically disordered, microtubule associated protein and is responsible for the promotion of microtubule (MT) formation and stabilization. The human Tau protein has six different isoforms, which are expressed ... -
Non-Canonical Amino Acids as Minimal Tags for Investigating Protein Organization and Turnover
(2016-01-08)The burgeoning field of genetic code expansion provides new tools for specifically labeling proteins for a variety of applications. Clickable non-canonical amino acids (ncAA) have been refined for almost-quantitative and ... -
Non-canonical functions of the MDM2 oncoprotein
(2022-11-04)The tumour suppressor gene TP53 is mutated in ~50% of human cancers. The p53 protein is a transcription factor which is induced by several cellular stresses, such as ribosomal stress and DNA damage. Expression of its target ... -
Non-equilibrium Statistical Mechanics Studied Through the Lens of Stochastic Calculus
(2024-01-11)The microscopic world of atoms and molecules is continuously in motion, which is manifested on the macroscopic scale as a non-zero temperature. Due to collisions with surrounding molecules that perform thermal motion, many ... -
Nonequilibrium Statistical Mechanics
(2020-03-27)Active matter is everywhere, from macroscopic to microscopic scales, we find systems such as human crowd or flock of birds as well as bacterial colonies. These systems composed of particles are able to convert their ... -
Nonlinearities in bipolar cells and their role for encoding visual signals
(2019-05-02)Vision begins in the retina, where ganglion cells separate the visual input into ~30 parallel output channels with different response characteristics to visual stimuli. How retinal ganglion cells obtain such a diversity ... -
Norepinephrine induces internalization of Kv1.1 in hippocampal neurons
(2017-08-08)Norepinephrine (NE), a catecholamine produced by dopamine β-hydroxylase, is released either as a hormone from the adrenal medulla into the blood, or as a neurotransmitter in the brain. Norepinephrine signaling serves as a ... -
Novel Applications of Super-Resolution Microscopy in Molecular Biology and Medical Diagnostics
(2016-03-31)Despite recent advances in microscopy techniques, the underlying labeling assays remained mostly unchanged. However, super-resolution techniques require other staining conditions than conventional light microscopy, like ... -
Novel approach for identification of biocatalysts by reverse omics techniques
(2019-08-06)A novel screening approach for identification of biocatalysts by reverse omics techniques was developed. Basic principle is the differential analysis of metatranscriptomes obtained from cultures treated either with the ... -
Novel approaches to measure physiological and behavioral responses to stress
(2020-02-21)Stress is the perception of threats to one's physical or psychological integrity, leading to certain autonomous, affective, and behavioral responses. Albeit its important role in adapting to new contexts and thus increasing ... -
Novel design and data analysis concepts for MINFLUX nanoscopy
(2021-11-22)MINFLUX is a novel concept for super-resolution fluorescence microscopy that achieves unprecedented image resolution despite the limited photon budget of fluorescent molecules. The goal of this thesis is to improve MINFLUX ... -
Novel export and import pathways in S. cerevisiae identified by an engineered SUMO system
(2018-09-07)Nuclear transport receptors (NTRs) mediate the translocation of cargos between the cytoplasm and the nucleus. NTRs are classified either as importins or exportins according to the directionality of the cargo transport. To ... -
Novel production system for influenza A virus-derived defective interfering particles and analysis of antiviral activity
(2020-09-24)Influenza A virus (IAV) increases morbidity and mortality rates and novel antivirals are needed to combat the virus. Errors of the viral polymerase lead to the generation of defective RNAs. These DI-RNAs may interfere with ...