Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Title
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PTK7 protein localization and stability is affected by canonical Wnt ligands
(2016-02-04)Wnt co-receptors have been shown to be endocytosed in response to Wnt proteins. However, the role of Wnt receptor internalization for Wnt signaling is a topic of ongoing research. Here we show that the Wnt co-receptor PTK7 ... -
PUCK: Primer Utilised CRISPR/Cas Knock-Ins for High Throughput Super-Resolution Microscopy
(2024-11-29)The insertion of tags into proteins via gene editing is a powerful tool for microscopy, enabling the comparative analysis of different proteins. The advent of CRISPR/Cas systems has greatly simplified gene editing and ... -
Pulvinar-cortical interactions for spatial perception and goal-directed actions in non-human primates
(2018-12-12)The pulvinar nucleus, the largest thalamic nucleus in primates, has been shown to be involved in goal-directed visuomotor behavior related to the contralateral side of visual space. However, there is conflicting evidence ... -
Purification of organellar macromolecular complexes for structural biology
(2022-03-14)Photosynthesis is the source of life on Earth. In oxygenic photosynthesis, water and carbon dioxide are converted into organic matter using of solar energy that results in the release of oxygen. PSII, a supra-molecular ... -
Quantification of forces involved in carbohydrate induced attachment of carcinoma cells
(2020-02-26)Vital biological processes such as the adhesion of cells to their surroundings rely on short-term recognition processes, which are defined by a rapid assembly and disassembly of adhesion molecules. These processes were ... -
Quantifying Evolutionary Dynamics
(2015-06-04)Adaptation is a central process in evolution: populations adapt to their environment by accumulating beneficial mutations over the course of generations. Microbial populations present an excellent experimental system that ... -
Quantifying the diffusion of membrane proteins and peptides
(2013-08-15)Lipid and protein diffusion in membranes is a fundamental requirement for many signaling processes in biological cells. Therefore, measuring protein and lipid mobility in lipid bilayers with high accuracy is essential for ... -
Quantifying topological transformation in lipid self-assemblies
(2021-06-03)Surfactant assemblies are characterized by soft intermolecular interactions and can be deformed or topologically altered by thermal or mechanical stress of the magnitude of thermal fluctuations. In biology, the protein ... -
Quantitation Strategies in Optically Sectioning Fluorescence Microscopy
(2009-06-15)The development of microscopes that can record fluorescence intensity in five and more dimensions in one experiment (e.g. space, time and colour) makes parameters available for the scientific ... -
Quantitative analysis of Förster resonance energy transfer from spectrally resolved fluorescence measurements
(2010-08-25)A novel method for spectral analysis of Forster resonance energy transfer (FRET) signals is presented. This method provides a general solution for donor quenching and acceptor sensitization ... -
Quantitative Modeling of RNA-Protein Interactions
(2021-05-07)RNA-binding proteins (RBPs) impact every aspect of RNA metabolism including RNA transcription, maturation, export, localization, translation, and stability. Specific RNA-protein interactions therefore play a central role ... -
Quantitative molecular physiology at active zones of calyceal synapses of the auditory pathway
(2023-11-17)Bushy cells (BCs) of the anteroventral cochlear nucleus (aVCN) are the first relay station of ascending auditory information. Sound is transformed from mechanical to electrical stimuli by the inner hair cells of the cochlea ... -
Quantitative Multi-Parameter Mapping in Magnetic Resonance Imaging
(2023-08-18)Magnetic resonance imaging (MRI) is a versatile imaging technology with a broad variety of biomedical and clinical applications. It provides an excellent soft tissue contrast without the need for ionizing radiation or ... -
Quantitative Multicolour Nanoscopy for the Investigation of the Postsynaptic Actin Cytoskeleton
(2023-08-16)Cognitive processes like the formation of memory and learning originate in the correct functioning of synapses. Dendritic spines are highly plastic, specialised actin-rich structures harbouring the postsynaptic site in ... -
Quantitative Nanoscopy of Synaptic Sites
(2023-08-23)Synapses are the basic information processing unit of the brain. Neuronal activity and memory formation thereby require a vastly dynamic and rapidly changing synaptic environment and composition. In order to function, these ... -
Quantitative phosphoproteomics for studying B-cell receptor signaling in Burkitt’s lymphoma
(2017-02-16)Protein phosphorylation plays an important role in transmitting cellular signals. Phosphorylation has been mostly characterized by hypothesis-driven and low-throughput approaches in the past. However, novel phosphorylation ... -
Rab26 mediates selective targeting of synaptic vesicles to the autophagy pathway
(2015-02-26)In recent years it has been shown that macroautophagy regulates the turnover of postsynaptic receptors and modulates presynaptic neurotransmission. However it is still unclear whether the presynaptic protein turnover is ... -
Reaction mechanism of hOMPD and CaAAD at atomic resolution
(2019-12-12)Decarboxylation reactions are fundamental processes in the chemistry of the animated world. Thus, the evolution of decarboxylases brought up a stunning array of diverse enzymes utilizing a variety of different mechanisms ... -
Reading memory traces in cultured neuronal networks by probabilistic analysis
(2014-02-21)In this thesis, I am studying the enhancement of the collective burst dynamics, burst activation and propagation in hippocampal neuronal networks in-vitro by using optogenetic techniques. Channelrhodopsin-2 transfected ... -
Receptive Field Characterization in MSTd Neurons
(2018-12-14)The dorsal region of the medial superior temporal area (MSTd) in primate extrastriate visual cortex is reported to play a major role in the encoding and perception of optic flow stimuli, i.e. large-scale motion patterns ...