Blättern GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften nach "Enderlein, Jörg Prof. Dr." Betreuer
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Advanced Image Deconvolution Techniques for Super-resolution Microscopy
(2019-09-12)In last decades, super-resolution fluorescence microscopy has seen its great development. Many super-resolution techniques are developed: the non-linear super-resolution techniques such as STED, PALM/STROM have provided ... -
Advances in enhanced multi-plane 3D imaging and image scanning microscopy
(2021-01-07)High-speed 3D optical microscopy is an indispensable requirement in studying rapid processes like signaling in neuronal networks, flagellar motion, or complex motion of living and highly dynamic subcellular components. ... -
Diffusion and Flow on Microscopic Length Scales Studied with Fluorescence Correlation Spectroscopy
(2012-10-30)The physics of fluids on microscopic lengthscales is fundamentally different from the one governing macroscopic fluid phenomena. On lengthscales up to a few micrometer, inertial forces of small particles and fluid elements are ... -
Extending Resolution in All Directions: Image Scanning Microscopy and Metal-induced Energy Transfer
(2019-03-18)Fluorescence microscopy is a powerful tool in the life sciences and is used to study structure and function on length scales from cells down to single molecules. In recent years, fluorescence microscopy has seen enormous ... -
Fluorescence Lifetime Single Molecule Localisation Microscopy
(2021-03-26)In conventional fluorescence microscopy, species are distinguished by colour. While these images provide an abundance of information about the sample, they are, in many aspects limited by artefacts and resolution, and some ... -
Fluorescence spectroscopic studies of protein conformational dynamics
(2013-10-31)The folding of a protein into its native structure is the fundamental prerequisite for its functionality. The polypeptide chain itself contains the chemical information necessary for spontaneous folding of the protein. ... -
Molecular Sizing using Fluorescence Correlation Spectroscopy
(2010-11-04)Size is one of the basic characteristics of any object and especially of molecules. It is directly and independently of other properties related to fundamental physical phenomena, like ... -
Nanoscale Brownian Dynamics of Semiflexible Biopolymers
(2020-07-24)Proteins are one of, if not the most, versatile of biology’s tools, performing a great variety of functions in living organisms and giving rise to behavior as complex as life itself. They are linear chains of amino acids ... -
Nanoscale Photonics
(2017-08-14)Detecting single nanometre scale objects and studying them in a controlled manner in solution is still a difficult task due to diffusion. We demonstrate one-dimensional nanofluidic flows of single molecules by confining ... -
Probing Molecular Stoichiometry by Photon Antibunching and Nanofluidics Assisted Imaging in Solution
(2017-07-05)A mechanistic understanding of biological function requires a quantitative determination of macromolecular subunit architecture and interaction. Optical microscopy and spectroscopy provide a noninvasive method to characterize ... -
Quantifying the diffusion of membrane proteins and peptides
(2013-08-15)Lipid and protein diffusion in membranes is a fundamental requirement for many signaling processes in biological cells. Therefore, measuring protein and lipid mobility in lipid bilayers with high accuracy is essential for ... -
Single Molecule Cryo-Fluorescence Microscopy
(2016-11-07)This work presents a newly developed cryo-fluorescence microscopy system and several of its applications. The thesis is divided into three parts. Part I introduces the design of the cryo-fluorescence microscope, and ... -
Single Molecule Fluorescence Spectroscopy and Imaging: Advanced Methods and Applications in Life Sciences
(2021-06-03)The visualization of biological structures down to the molecular length scale has been recently made possible by the development of super-resolution fluorescence microscopy. These techniques now routinely resolve biological ... -
Single molecule fluorescence spectroscopy of the structure and dynamics of the spliceosome
(2013-11-05)Binding reactions can be ideally investigated by Dual-Color Fluorescence Cross-Correlation Spectroscopy (FCCS). Here, this method is used to study the recruitment and dissociation of proteins to and from the spliceosome. ... -
Stochastic Optical Fluctuation Imaging - Labels and Applications
(2015-05-26)Superresolution fluorescence imaging is an important tool for studying subcellular structures and processes. A technique with great potential is stochastic optical fluctuation imaging (SOFI), a fluorescence imaging modality ... -
Study Conformational Dynamics of Intrinsically Disordered Proteins by Single‐Molecule Spectroscopy
(2016-09-06)Intrinsically disordered proteins (IDPs) are proteins which lack a well-defined three- dimensional structure. The abundance and functional significance of IDPs has been recognized only recently. Due to their properties, ...