Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Advisor "Jahn, Reinhard Prof. Dr."
Now showing items 1-14 of 14
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A Quantitative Mass Spectrometric Analysis of Signalling Proteins in Human Sperm Cells
(2024-05-17)Fertilization of an oocyte by a sperm cell is a complex multi-stage process. First, a sperm cell must locate and reach the oocyte; second, it must undergo capacitation, the acrosome reaction, and hyperactivation to penetrate ... -
A Single Vesicle Assay to Study the Electrochemical Gradient Regulation in Glutamatergic and GABAergic Synaptic Vesicles
(2016-02-24)During neuronal activity, synapses sustain neurotransmission by a high fidelity multi-step process called synaptic vesicle (SV) recycling. This process involves endocytosis, neurotransmitter loading and exocytosis of SVs ... -
Biochemical characterization of presynaptic membrane protein complexes
(2017-09-13)At chemical synapses, synaptic vesicles, membrane-enclosed entities filled with neurotransmitters, undergo exocytosis at restricted areas of the presynaptic membrane called active zones (AZ). The core AZ proteins form a ... -
Development of a versatile microfluidic platform to study the ionic mechanisms of vesicular neurotransmitter transporters
(2021-11-04)This thesis described the development of a novel, versatile microfluidic platform for studying the ionic mechanisms of vesicular neurotransmitter transporters in rodent synaptic vesicles (SVs). The level of filling of an ... -
In vitro investigation of trans SNARE complexes arrested between artificial membranes
(2015-09-23)The key components of the machinery that catalyzes membrane fusion include membrane-associated small proteins that are termed as soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). Calcium-triggered ... -
Investigation of SNARE function in the early endosomal compartment
(2009-12-07)Soluble N-ethylmaleimide-sensitive factor attachment receptor (SNARE) proteins catalyze organelle fusion in the secretory pathway. Di erent fusion steps are mediated by speci.c SNARE sets. ... -
Kinetic analysis of neuronal SNARE protein interactions
(2022-08-08)When an action potential arrives at the axon terminal, it causes a local and transient increase in the Ca2+ concentration that starts a cascade of molecular interactions eventually leading to the fusion of synaptic vesicles ... -
Molecular profiling of presynaptic docking sites
(2011-10-20)In presynaptic nerve endings, exocytosis of synaptic vesicles is restricted to specialized areas of the plasma membrane, called active zones, that are distinguished by electron-dense material. Here synaptic vesicles ... -
Nanoscale organization and dynamics of SNARE proteins in the presynaptic membranes
(2015-10-19)The specific organization of proteins and lipids in functional domains in biological membranes allows localization and segregation of specific physiological activities. Mechanisms that underlie the formation of these domains ... -
Rab26 mediates selective targeting of synaptic vesicles to the autophagy pathway
(2015-02-26)In recent years it has been shown that macroautophagy regulates the turnover of postsynaptic receptors and modulates presynaptic neurotransmission. However it is still unclear whether the presynaptic protein turnover is ... -
Reconstituted SNARE-mediated fusion: towards a mechanistic understanding
(2012-03-20)A novel and well-characterized model system has been developed and used in order to extract mechanistic insights on the SNARE-mediated fusion process. The reconstitution of SNAREs onto large ... -
Regulation of the neuronal SNARE-complex by accessory proteins
(2018-02-16)In this work, I have thoroughly characterized a novel complex containing syntaxin1a (1-288), SNAP25a and Munc18-1 and established it as an efficient acceptor complex for synaptobrevin-binding, using several biochemical and ... -
Study of SNARE-mediated membrane fusion with a novel single vesicle fusion assay
(2018-06-12)Membrane fusion in the secretory pathway of eukaryotic cells, including exocytosis of synaptic vesicles, is catalysed by SNARE proteins. In case of the synaptic vesicle fusion, two of the members of this protein family ... -
Targeting ion sensors to synaptic vesicles using a Nanobody against luminal Synaptotagmin 1
(2022-02-24)The synapse is a specialized nanostructure that connects two neurons where the ionic composition in each of its nano compartments is the driving process for synaptic transmission. Synaptic transmission relies on the ...