Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Griesinger, Christian Prof. Dr."
Now showing items 1-20 of 21
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A twist in NMR relaxation experiments: Application to the study of protein motions
(2019-09-27)Understanding biological processes requires knowledge about the structure as well as dynamics of the involved molecules. Proteins are complex structures, which experience time dependent changes due to atomic motions. The ... -
Characterization of the Munc13 - CaM Interaction
(2009-07-06)The Munc13 proteins are the key mediators of synaptic vesicle priming, an essential step in Ca2+-regulated neurotransmitter release that renders docked vesicles fusion-competent prior to ... -
Development and Application of CP-ENDOR Spectroscopy on Protein Radicals
(2020-03-09)Electron-nuclear double resonance (ENDOR) spectroscopy is the method of choice for detecting magnetic nuclei in biomolecules which contain an unpaired electron spin. However, due to its low gyromagnetic ratio, the detection ... -
Development of 13C Liquid State Dynamic Nuclear Polarization at 9.4 Tesla
(2023-07-11)Throughout the last decades, nuclear magnetic resonance (NMR) spectroscopy in liquids contributed to the structural elucidation of molecules of many types and sizes. Continuous improvements of the hardware and pulse sequences ... -
From Paramagnetic Proteins to Field Alignment of Small Molecules
(2022-03-17)In this dissertation we show how to push the boundaries of structure elucidation using tensorial NMR parameters. Possible sources for these effects are paramagnetic centers or molecular alignment, and they contain valuable ... -
Identification and Analysis of Gene Product Modifiers of α-Synuclein Toxicity in the Fruit Fly (D. Melanogaster).
(2010-09-22)Parkinson s disease (PD) is a progressive neurodegenerative disorder, pathologically characterized by loss of dopaminergic neurons in the substantia nigra pars compacta. Various mutations ... -
Import of Presequence-Containing Precursor Proteins into Mitochondria
(2014-07-07)The presequence pathway is utilized by over 70% of all cytosolically translated proteins destined to the mitochondria, emphasizing the significance imparted by presequence import on mitochondria translocation. Presequence ... -
Molecular Dynamics Studies of Permeation and Gating Mechanisms in Potassium Channels
(2023-04-19)Potassium (K+) channels contribute to diverse physiological activities, representing an indispensable part of living organisms. A thorough understanding of different aspects of permeation mechanisms and gating in K+ channels ... -
On the Effect of Binding on Ubiquitin Dynamics
(2014-03-26)Protein-protein interactions play an important role in all biological processes. But the principles underlying these interactions are not yet fully understood. Ubiquitin is a small signalling protein that non-covalently ... -
On the molecular basis of α-synuclein aggregation on phospholipid membranes in the presence and absence of anle138b
(2020-07-22)A physiologic hallmark of synucleinopathies such as Parkinson’s Disease (PD) or Multiple System Atrophy (MSA) are deposits in patients’ brains, which consist of the α-synuclein. Growing evidence suggests, that oligomeric ... -
Paramagnetic Tagging of Oligonucleotides for Structure Determination using NMR-Spectroscopy
(2015-03-31)Structure determination is one of the most fundamental fields of research, as it provides insights in biological systems and their mechanisms. Therein, NMR spectroscopy is an essential tool for investigations under ... -
Phase Separation Studied by Nuclear Magnetic Resonance
(2024-01-04)Signal transduction by the ligated B cell receptor (BCR) depends on the pre-organization of its intracellular components, such as the effector proteins SLP65 and CIN85 within phase-separated condensates. These liquid-like ... -
Sensitivity Enhanced NMR
(2014-04-16)The topic of this thesis is the investigation of how sensitivity enhanced Nuclear Magnetic Resonance (NMR) spectra of biological systems could be obtained by dynamic nuclear polarization (DNP) under conditions close to ... -
Structural and Functional Investigations of Transmembrane Signaling Histidine Kinase Using NMR
(2022-11-04)Membrane proteins play a central role in biology as the gatekeepers of all cellular compartments. And transmembrane signaling through membrane bound kinases is a particularly important process for cellular adaptation to a ... -
Structure and biochemistry of polyunsaturated fatty acid double bond isomerase from Propionibacterium acnes
(2007-12-06)Conjugated linoleic acid (CLA) is a collective term describing positional and geometrical isomers of linoleic acid (LA, 18:2delta9Z,delta12Z). This class of compounds is receiving much ... -
Studying Molecular Interactions under Flow with Fluorescence Fluctuation Spectroscopy
(2020-06-11)Assembly and interactions of biomolecules are fundamental processes in living organisms to maintain the physiological cellular behavior. The majority of the time protein assembly is useful and vital for the organism. ... -
<sup>17</sup>O hyperfine spectroscopy to investigate water binding to organic radicals
(2022-07-22)Hyperfine spectroscopy experiments detect nuclear spins around paramagnetic centers. They reveal magnetic interactions that contain valuable structural information on distance and orientation of the coupling partners. One ... -
Systems biology in Bacillus subtilis
(2010-11-16)Systems biology studies the way in which interaction of different types of molecules results in complex behaviour at the cellular, organism, and higher levels. It approaches these questions ... -
The Advantages Of Paramagnetic NMR
(2013-11-28)There are three distinct effects in nuclear magnetic resonance spectroscopy (NMR) that differ between paramagnetic and diamagnetic molecules in isotropic solution. These are residual dipolar coupling (RDC), pseudocontact ... -
Towards Uncovering the Role of Pre-fibrillar Oligomers of á-Synuclein in the Pathogenesis of Parkinson's Disease
(2010-08-06)Parkinson s disease (PD) is a progressive neuropathological disorder that is characterized by the presence of intra-cytoplasmic inclusions called Lewy Bodies (LBs). LBs represent insoluble ...