Dokumente GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften nach Gutachter "Hell, Stefan Prof. Dr."
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Aberration correction in STED microscopy
(2019-11-21)In the last decade, superresolution microscopy techniques became a valuable tool to study sub-cellular structures with a resolution below the diffraction limit. The use of superresolution for brain research requires to ... -
Automated STED microscopy for cell-biological high-throughput assays
(2022-11-28)In the past decade, super-resolution fluorescence microscopy has revolutionized biological research by enabling the study of biological functions down to the molecular scale. Despite profound technological developments, ... -
Engineering of a NIR fluorescent protein for live-cell nanoscopy
(2020-09-22)Since its first use as a genetically encoded fluorescent marker in 1994, GFP and its homologues have fundamentally revolutionized live-cell fluorescence imaging and became an essential tool for biomedical research. Many ... -
Microfluidic cryofixation for time-correlated live-imaging cryo-fluorescence microscopy and electron microscopy of Caenorhabditis elegans
(2018-11-01)Light and electron microscopy are complementary methods to study biological systems at the cellular and sub-cellular scale. Light microscopy is compatible with live cells, allowing features of interest to be selectively ... -
Molecular physiology of synaptic sound encoding at the first auditory synapse
(2018-03-20)To achieve accurate encoding of sounds, inner hair cell (IHC) ribbon-type synapses are highly specialized to release synaptic vesicles (SVs) with high rates and temporal precision. A sophisticated, by far not yet fully ... -
Nanoscale organization and dynamics of SNARE proteins in the presynaptic membranes
(2015-10-19)The specific organization of proteins and lipids in functional domains in biological membranes allows localization and segregation of specific physiological activities. Mechanisms that underlie the formation of these domains ... -
Nanoscale probing of single synapse function and BDNF Cell-to-Cell transfer
(2016-12-22)Brain-derived neurotrophic factor (BDNF) is one of the key modulator/mediator molecules for synaptic plasticity in the adult nervous system and also coordinates neural development, survival, differentiation and axon growth ... -
Nanoscopy inside living brain slices
(2014-10-30)In order to understand how memory is processed and stored in the brain, it would be helpful to observe the ongoing memory processes in action. This is no easy task, however, if it is to be done at the synaptic level. It ... -
Quantitative Multicolour Nanoscopy for the Investigation of the Postsynaptic Actin Cytoskeleton
(2023-08-16)Cognitive processes like the formation of memory and learning originate in the correct functioning of synapses. Dendritic spines are highly plastic, specialised actin-rich structures harbouring the postsynaptic site in ... -
Quantitative Nanoscopy of Synaptic Sites
(2023-08-23)Synapses are the basic information processing unit of the brain. Neuronal activity and memory formation thereby require a vastly dynamic and rapidly changing synaptic environment and composition. In order to function, these ... -
RESOLFT nanoscopy with water-soluble synthetic fluorophores
(2018-01-17)Fluorescence microscopy is an important and widely used tool in the life sciences due to its unique ability to observe cellular processes in living specimens with target-specific image contrast. The development of high ... -
STED Microscopy of FRET Pairs
(2018-05-18)Förster resonance energy transfer (FRET) is a popular tool in life sciences, for example to detect protein-protein interactions and ligand binding, or to construct fluorescent biosensors for metabolites or ions. Obtaining ... -
STED Microscopy with Scanning Fields Below the Diffraction Limit
(2016-11-17)Since its development, STED microscopy has been used extensively for imaging biological samples. To label structures of interest, fluorescent proteins and organic dyes are used almost exclusively. However, these labels ... -
STED nanoscopy of the living brain
(2012-05-03)Over the past decade, a new class of fluorescence microscopes has evolved which thoroughly break the diffraction limit of classical far-field light microscopy and thus provide significantly improved spatial resolution. ... -
Studying Protein Organization in Cellular Membranes by High-Resolution Microscopy
(2014-10-09)Most membrane proteins are found in clusters in the plasma membrane. For abundant proteins it is apparent that the clusters also tend to have patterned distributions, rather than being randomly scattered. To reveal the ...