Dokumente GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften nach Gutachter "Jahn, Reinhard Prof. Dr."
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A comprehensive characterization of brain and retina synaptic vesicle proteome by quantitative mass spectrometry.
(2017-06-07)Synaptic vesicles (SVs) are essential organelles for the transfer of information between a pre-synaptic nerve terminal and a post-synaptic target. SVs release their neurotransmitter content into the synaptic cleft in a ... -
Biochemical characterization of presynaptic membrane protein complexes
(2017-09-13)At chemical synapses, synaptic vesicles, membrane-enclosed entities filled with neurotransmitters, undergo exocytosis at restricted areas of the presynaptic membrane called active zones (AZ). The core AZ proteins form a ... -
Biochemical reconstitution of human autophagy initiation
(2023-05-04)Autophagy is a tightly regulated process that eukaryotic cells use as a major survival mechanism to reallocate nutrients to essential processes in adverse conditions such as nutrient or energy deprivation. Autophagy is ... -
Cellular function and toxicity of the Parkinson’s disease-related genes α-synuclein and catp-6 in C. elegans
(2013-04-09)Parkinson’s disease (PD) is the second most common neurodegenerative disease in industrialized countries. However, the molecular mechanisms leading to neuronal death in PD still remain unclear. About 5-10% of PD patients ... -
Characterization of the Role of <i>aeneas</i> in Primordial Germ Cell Migration and Blastoderm Cellularization
(2007-09-25)The communication and mutual influence of epithelia and migratory cells is a field of high scientific and medical relevance. Many cellular processes underlying the establishment of cell ... -
Dense-core vesicle maturation at the Golgi-endosomal interface in Caenorhabditis elegans
(2012-06-08)Fast synaptic transmission is mediated by the triggered release of neurotransmitters from synaptic vesicles (SVs). To regulate synaptic transmission and neuronal activity, neurons also release neuropeptides and hormones ... -
Development of Fluorescence Activated Synaptosome Sorting (FASS) and analysis of VGLUT1 synapses from mouse brain
(2012-10-05)Signal propagation between neurons in the brain is mediated by chemical synapses. Depending on the neurotransmitters and their receptors, synapses can transmit excitatory, inhibitory, or modulatory signals. A detailed ... -
Development of new methods in fluorescence microscopy
(2015-12-02)In recent years, advancements in single-molecule imaging techniques have enabled scientists to study in great detail the cells and relevant physiological processes, including neurons and the communication between them. ... -
Effects of arginine derivatives and oligopeptides on the physical properties of model membranes
(2021-02-16)Cell-penetrating peptides (CPPs) are often rich in arginine. They are able to cross cell membranes and carry other molecules into cells without being cytotoxic. The mechanisms by which they accomplish this are still ... -
Functional characterization of npcRNAs - Intercellular trafficking of generegulatory components via exosomes
(2017-04-11)Exosomes are small extracellular vesicles (EVs) of endocytic origin and get released by a multitude of cell types. These vesicles have been found in a variety of body fluids and more recently an important role in exchanging ... -
In vitro investigation of trans SNARE complexes arrested between artificial membranes
(2015-09-23)The key components of the machinery that catalyzes membrane fusion include membrane-associated small proteins that are termed as soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). Calcium-triggered ... -
Investigation of Endosomal Recycling of Synaptic Vesicles
(2010-11-05)During communication at chemical synapses, small neurotransmitter-filled synaptic vesicles, housed in the pre-synaptic nerve terminal (bouton), fuse with the plasma membrane (exocytosis) ... -
Kinetic analysis of neuronal SNARE protein interactions
(2022-08-08)When an action potential arrives at the axon terminal, it causes a local and transient increase in the Ca2+ concentration that starts a cascade of molecular interactions eventually leading to the fusion of synaptic vesicles ... -
Long-Term Temporal Dynamics of Synaptic Vesicles
(2017-09-11)Neurotransmission requires the release of neurotransmitters from synaptic vesicles. This occurs via fusion of the vesicle to the pre-synaptic membrane upon stimulation. However, not all synaptic vesicles are equally ... -
Mechanism of regulation of spliceosome activation by Brr2 and Prp8 and links to retinal disease
(2013-11-12)Splicing is a crucial post-transcriptional processing event that entails the removal of non-coding intervening sequences (introns) from eukaryotic pre-mRNA and the ligation of the coding sequences (exons). It is carried ... -
Mitochondrial adaptation at the neuronal presynapse
(2021-11-22)Synaptic transmission poses a major energy consuming process in the brain, but how neurons maintain a constant energy supply during extended synaptic activity and how presynaptic mitochondria contribute to the energy ... -
Molecular and Morphological Correlates of Synaptic Vesicle Priming
(2014-10-27)Excitation-secretion coupling at nerve cell synapses is a sub-millisecond process that entails the transduction of an electrical stimulus into synaptic vesicle fusion. Before fusion, synaptic vesicles are physically docked ... -
Molecular Mechanisms of Astrocyte Vesicle Fusion at Synaptic Interfaces
(2015-12-01)Astrocytes are key to neuronal trophic support, development, and synaptic signalling and plasticity. Astrocytes can detect synaptic events at tripartite synapses –a major site of bidirectional communication between neurones ... -
Molecular mechanisms of collybistin-dependent gephyrin clustering at inhibitory synapses
(2014-07-03)Information processing and transmission in neuronal networks in the mammalian brain occurs through intercellular communication between neurons at synapses. Inhibitory synapses play a key role, for example to maintain network ... -
Molecular mechanisms of synaptic vesicle recycling with a focus on Endophilin A and Rabconnectin-3a
(2019-11-01)Synaptic vesicle (SV) recycling at the synapse is fundamental to neurotransmission and it sustains unimpaired neuronal communication. It is a complex cell biological process, where the molecular steps are finely coordinated ...