Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Rodnina, Marina Prof. Dr."
Now showing items 1-20 of 30
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Biochemical reconstitution of human autophagy initiation
(2023-05-04)Autophagy is a tightly regulated process that eukaryotic cells use as a major survival mechanism to reallocate nutrients to essential processes in adverse conditions such as nutrient or energy deprivation. Autophagy is ... -
Co-translational processing and membrane insertion of proteins in bacteria
(2022-11-04)Protein biogenesis entails the synthesis of polypeptides on the ribosome, their co- and post-translational maturation, folding, and, in the case of membrane proteins, insertion into the lipid bilayer. Bacterial proteins ... -
Cotranslational folding of HemK C-terminal domain
(2023-03-17)The correct folding of proteins is essential for the survival of the cell. Despite the importance of the question, it remains largely unclear how proteins, in particular large multidomain proteins with mixed α-helical and ... -
Crystal Structure and Characterization of the SCOC Coiled Coil Domain
(2014-08-06)Autophagy is a conserved eukaryotic process for the degradation of cytosolic content. An expanding double layered membrane takes up part of the cytoplasm and matures to an autophagosomal vesicle. The autophagosome ... -
Development, characterization, and application of RNA catalysts for in situ labeling of target RNA molecules
(2020-08-07)RNA molecules play a diverse set of crucial roles in biological systems. Studying various aspects of RNA biology such as RNA transport, localization, degradation, and structural dynamics is therefore of utmost significance. ... -
Dynamic interactions during ribosome targeting to the membrane
(2015-05-11)The signal recognition particle (SRP) and its receptor are the main components of the co-translational protein targeting pathway by which membrane proteins are targeted to the membrane. The co-translational targeting pathway ... -
Functional analyses of RNA helicases in human ribosome biogenesis
(2019-10-30)Eukaryotic ribosomes are essential ribonucleoprotein (RNP) complexes consisting of four ribosomal RNAs (18S, 5S, 5.8S, 28S/25S) and approximately 80 ribosomal proteins, which are required for the process of translating the ... -
Functional characterization and application of 2',5'- branched RNA forming deoxyribozymes using lanthanides as cofactors
(2013-12-13)Deoxyribozymes or DNA catalysts are synthetic single stranded DNA molecules capable of catalysis for a wide range or reactions. Deoxyribozymes are discovered by in vitro selection and are mostly dependent on bivalent metal ... -
Identification of nuclear export signals and structural analysis of transport complexes.
(2013-01-09)The nucleus is a vital sub cellular compartment and it stores the genome. Transport across the nuclear envelope occurs through Nuclear Pore Complexes (NPC) and cargo traffic across the NPCs is a highly specific and ... -
Import of proteins along the presequence pathway
(2017-12-01)The aim of this study was to investigate the mechanism of protein transport by the presequence translocase (TIM23) in the inner mitochondrial membrane. Presequencecontaining proteins are imported through the translocase ... -
Insights into the biogenesis of the human mitochondrial ribosomal large subunit – Characterisation of mL44 and mL45
(2020-11-16)Due to the presence of high-resolution cryo-EM structures of the human mitoribosome, the understanding about the function and assembly of the intriguing mitochondrial ribonucleoprotein complex increased tremendously during ... -
Kinetic analysis of neuronal SNARE protein interactions
(2022-08-08)When an action potential arrives at the axon terminal, it causes a local and transient increase in the Ca2+ concentration that starts a cascade of molecular interactions eventually leading to the fusion of synaptic vesicles ... -
Mechanism of Ribosome Rescue by Alternative Release Factor B
(2020-07-09)Rescue of ribosomes stalled on non-stop mRNAs is essential in bacteria and in the mitochondria of all eukaryotes. The lack of a stop codon causes ribosomes to stall at the 3’ end of mRNA with an empty A site, and specialized ... -
Mechanistic Characterization of Transaldolase from <i>Thermoplasma Acidophilum</i> and Preliminary Analysis of the QncN/L-M Protein System from <i>Streptomyces Melanovinaceus</i>
(2018-02-13)The results introduced in the present work are mainly based on the pre-steady state and steady-state analysis supported by structural information from crystallographic studies. Different variants of TacTAL were generated ... -
Molecular mechanism of selenocysteine incorporation in bacterial translation
(2012-06-27)Selenocysteine is the 21st amino acid which is incorporated into proteins by recoding a stop codon UGA followed by a selenocysteine insertion sequence (SECIS) of the mRNA. In bacteria, selenocysteine insertion requires ... -
Monitoring the late events of translation initiation in real-time
(2016-10-19)In bacteria, translation initiation is promoted by three initiation factors (IF1, IF2 and IF3) and entails three distinct steps. First, the small ribosomal subunit (30S) recruits the initiation factors, initiator tRNA ... -
Novel export and import pathways in S. cerevisiae identified by an engineered SUMO system
(2018-09-07)Nuclear transport receptors (NTRs) mediate the translocation of cargos between the cytoplasm and the nucleus. NTRs are classified either as importins or exportins according to the directionality of the cargo transport. To ... -
Programmed Translational Readthrough in Drosophila melanogaster
(2020-06-05)Translational readthrough (TR) is extensively used by viruses to expand their limited genome capacity. The pervasiveness of TR across eukaryotic genomes is only recently being explored. Systems biology approaches such as ... -
Recoding of bacteriophage T4 gene 60 mRNA by programmed translational bypassing
(2020-07-28)During synthesis of a topoisomerase subunit encoded by the gene 60 of bacteriophage T4, the ribosome takes off from a certain codon, slides over a 50-nt non-coding segment of mRNA and resumes translation on a specific ... -
Recoding of viral mRNAs by –1 programmed ribosome frameshifting
(2019-06-11)–1 programmed ribosome frameshifting (–1PRF) is commonly used in many viruses and cellular genes. The current study focuses on the –1PRF in two human viruses, human immunodeficiency virus type 1 (HIV-1) and Semliki Forest ...