Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Tittmann, Kai Prof. Dr."
Now showing items 1-20 of 54
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Accessing the kinetics of the supra-tauc range via relaxation dispersion NMR spectroscopy
(2013-10-30)Nuclear Magnetic Resonance spectroscopy (NMR) is capable of attaining dynamic information of motions over a broad range of time scales under physiological relevant conditions while maintaining atomic resolution. Of the ... -
Biochemical analysis of CYP74-enzymes in Physcomitrella patens
(2014-03-18)In this study recombinant CYP74-enzymes AOS1, AOS2 and HPL from the moss P. patens, DES from S. tuberosum and EAS from the lancelet B. floridae were expressed in E. coli and purified for further analysis. PpAOS1 and BfEAS ... -
Biochemical Characterization of Human Guanylate Kinase and Mitochondrial Thymidine Kinase: Essential Enzymes for the Metabolic Activation of Nucleoside Analog Prodrugs
(2015-07-28)Deoxyribonucleoside and nucleotide kinases are key enzymes that catalyze the critical phosphorylation steps in the conversion of antiviral and anticancer nucleoside analogs to their corresponding cytotoxic nucleoside ... -
Catalysis at the Interface- Elucidation of the Activation Process and Coupling of Catalysis and Compartmentalization of the Peripheral Membrane Protein Pyruvate Oxidase from Escherichia coli
(2014-02-06)The thiamine- and flavin-dependent peripheral membrane protein pyruvate oxidase from E. coli (EcPOX) catalyzes the oxidative decarboxylation of the central metabolite pyruvate to CO2 and acetate with a concomitant electron ... -
Characterizing the Structure and Mechanics of 2D Clathrin Lattices with Atomic Force Microscopy
(2015-10-30)Clathrin is a self-assembling protein involved in intracellular trafficking. Since its discovery in the early 1960s, much has been learned about its structure, assembly, and regulation mechanisms. The three legged monomers, ... -
Co-translational processing and membrane insertion of proteins in bacteria
(2022-11-04)Protein biogenesis entails the synthesis of polypeptides on the ribosome, their co- and post-translational maturation, folding, and, in the case of membrane proteins, insertion into the lipid bilayer. Bacterial proteins ... -
Computational methods for the structure determination of highly dynamic molecular machines by cryo-EM
(2019-02-20)In the last couple of years, electron cryomicroscopy (cryo-EM) has gained of rising importance in the field of structural biology and biophysics. Not only that the routinely achievable resolution of the method has ... -
Computational study of the molecular details of ion permeation across the formate-nitrite transporters
(2016-07-12)The selective flux of molecules across biological membranes is essential for the normal function of any cell, and is governed by diverse membrane transport proteins. Historically, the focus in the membrane transport field ... -
Conformational Dynamics of large protein Complexes
(2015-02-18)Life on earth is only possible through the enormous capabilities of proteins and their assemblies. Which function a certain protein fulfills is encoded in its amino acid sequence, which gives rise to a defined structure. ... -
Cotranslational folding of HemK C-terminal domain
(2023-03-17)The correct folding of proteins is essential for the survival of the cell. Despite the importance of the question, it remains largely unclear how proteins, in particular large multidomain proteins with mixed α-helical and ... -
Coupling of GTP hydrolysis by EF-G to tRNA and mRNA translocation through the ribosome
(2013-09-20)Translocation is the concerted movement of the messenger RNA (mRNA) and the transfer RNA (tRNA) through the ribosome. Although spontaneous in vitro, this process is catalyzed by the translational GTPase elongation factor ... -
Crystallization and biophysical characterization of spliceosomal protein complexes
(2015-02-20)mRNA splicing is a highly dynamic process by which non-coding sequences are removed from a pre-mature mRNA. It is catalyzed by the spliceosome, a huge and highly dynamic ribonucleoprotein complex consisting of up to five ... -
Effects of arginine derivatives and oligopeptides on the physical properties of model membranes
(2021-02-16)Cell-penetrating peptides (CPPs) are often rich in arginine. They are able to cross cell membranes and carry other molecules into cells without being cytotoxic. The mechanisms by which they accomplish this are still ... -
Elongation factor P: mechanism of action and opportunities for drug design
(2023-07-24)EF-P is a conserved protein that has shape similarity to the tRNAs and binds to the E site of the ribosome. EF-P enhances the rate of peptide bond formation during the synthesis of polyPro sequences. In E. coli one third ... -
Exploration of cargo spectrum and NES patterns recognized by the exportin CRM1
(2014-10-15)The nuclear envelope (NE) subdivides eukaryotic cells into a nuclear and a cytoplasmic compartment, forcing material exchange between these two compartments to proceed through the nuclear pore complexes (NPCs). While ... -
Exploring the human Hsp70-Hop-Hsp90 system for Tau recognition
(2022-03-22)Molecular chaperones are diligent helpers of the cell that ensure a well-balanced proteome by regulating protein production, protein folding, refolding and holding as well as protein degradation. In doing so, molecular ... -
Functional characterization and application of 2',5'- branched RNA forming deoxyribozymes using lanthanides as cofactors
(2013-12-13)Deoxyribozymes or DNA catalysts are synthetic single stranded DNA molecules capable of catalysis for a wide range or reactions. Deoxyribozymes are discovered by in vitro selection and are mostly dependent on bivalent metal ... -
Functional characterization of transketolase-like proteins and related model systems with respect to thiamin diphosphate mediated chemistry
(2014-11-12)Transketolases (TKTs, E.C 2.2.1.1) are ubiquitous distributed enzymes among all three domains of life. TKTs require thiamin diphosphate (ThDP) and bivalent cations as catalytic cofactors. They fulfill their catalytic ... -
Functional dynamics of the anti-HIV lectin OAA and NMR methodology for the study of protein dynamics
(2016-01-26)Specific recognition between biomolecules is at the basis of all biological processes, and a mechanistic description of molecular recognition is crucial for a detailed understanding of these processes. Two limiting models ... -
Interaction of the SecYEG translocon with the SRP receptor and the ribosome
(2015-05-08)This work aimed at studying the interaction between SecYEG and FtsY and before and after the translating ribosome has been transferred to the SecYEG translocon. In this study we have used monomers of the SecYEG complex ...