Browsing GGNB - Göttinger Graduiertenzentrum für Neurowissenschaften, Biophysik und molekulare Biowissenschaften by Referee "Zweckstetter, Markus Prof. Dr."
Now showing items 1-20 of 20
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Crystal Structure and Characterization of the SCOC Coiled Coil Domain
(2014-08-06)Autophagy is a conserved eukaryotic process for the degradation of cytosolic content. An expanding double layered membrane takes up part of the cytoplasm and matures to an autophagosomal vesicle. The autophagosome ... -
Exploring the human Hsp70-Hop-Hsp90 system for Tau recognition
(2022-03-22)Molecular chaperones are diligent helpers of the cell that ensure a well-balanced proteome by regulating protein production, protein folding, refolding and holding as well as protein degradation. In doing so, molecular ... -
Fullerene-Nitroxide Derivatives as Potential Polarizers for Dynamic Nuclear Polarization (DNP) in Liquid State
(2016-09-12)Nuclear magnetic resonance spectroscopy and imaging (NMR/MRI) are widely applied methods for non-destructive analysis of biological systems, materials and medical applications. Dynamic nuclear polarization (DNP) is a method ... -
Functional dynamics of the anti-HIV lectin OAA and NMR methodology for the study of protein dynamics
(2016-01-26)Specific recognition between biomolecules is at the basis of all biological processes, and a mechanistic description of molecular recognition is crucial for a detailed understanding of these processes. Two limiting models ... -
Investigating phase separation mechanisms for transcriptional control
(2020-11-13)Transcription of protein-coding genes by RNA polymerase (Pol) II is a highly coordinated process. In metazoan cells, transcription is regulated both at the initiation step by recruitment of the Pol II machinery as well as ... -
Mechanistic insights into alpha-Synuclein neuronal toxicity: misfolding, serine phosphorylation and interactions with Rab GTPases
(2013-11-18)Alpha-Synuclein(aS), a protein abundant in presynaptic neurons, has been found as the major component of Lewy bodies, the neuronal inclusion identified as the hallmark of Parkinson’s disease (PD). aS adopts intrinsically ... -
Molecular Mechanisms of Tau Protein Aggregation Inhibition
(2013-11-11)Of all neurodegenerative diseases, Alzheimer´s Disease is the most widespread dementia syndrome, exhibiting progressive memory loss and intellectual abilities. One of the pathological hallmarks of this disease is associated ... -
Neurotoxicity and aggregation of β-synuclein and its P123H and V70M mutants associated with dementia with Lewy bodies
(2019-06-14)Dementia with Lewy bodies is a progressive degenerative brain disorder which accounts for 7 to 25% of all dementia cases. Two rare missense mutations of β-synuclein, P123H and V70M were identified in familial and sporadic ... -
New sample preparation techniques of macromolecular complexes for high resolution structure determination using cryo-EM
(2020-04-07)Fatty acids are one of the most abundant lipids in the cell. Cells use them to build biological membranes, as energy reserves and as signaling molecules. Fatty acids are synthesized by a specialized protein machinery called ... -
NMR Spectroscopy of the Tau-Microtubule Interaction
(2015-12-10)Tau is an intrinsically disordered, microtubule associated protein and is responsible for the promotion of microtubule (MT) formation and stabilization. The human Tau protein has six different isoforms, which are expressed ... -
Optimization and application of Trim-Away for studying a liquid-like spindle domain in mammalian oocytes
(2019-10-01)In this thesis, I reported a membraneless structure that permeated a major region of the female meiotic spindle and formed droplet-like protrusions around the spindle poles. Proteins within this previously unknown structure ... -
Pharmacologically controlled neurotrophic factor gene therapy for Parkinson's disease
(2019-07-24)During the PhD thesis, two projects were carried out. In the first project, an inducible AAV-5 vector for expression of GDNF, under control of the approved small molecule drug mifepristone, was evaluated in rats. GDNF ... -
Solution NMR-based characterization of the structure of the outer mitochondrial membrane protein Tom40 and a novel method for NMR resonance assignment of large intrinsically disordered proteins
(2014-05-08)This dissertation is composed of two parts. In the first part, the characterization of the structure of liposome-embedded Tom40 using a novel hydrogen/deuterium (H/D) exchange method is described. In the second part, a new ... -
Structural studies of RNA polymerase II complexes with human TFIIS and capping enzymes
(2023-12-14)Transcription is a conserved fundamental cellular process carried out by RNA polymerases (Pol) that results in the synthesis of an RNA transcript. Transcription of protein coding genes by Pol II is the first step in ... -
Structure-based mechanistic analysis of the proteasome
(2019-05-02)The ubiquitin proteasome pathway is one of the major protein degradation pathways in the cell. This pathway is essential for protein quality control and the regulation of the cell cycle. Proteins subjected to the ubiquitin ... -
Systematic comparison of the molecular mechanisms underlying the spreading of pathology in different neurodegenerative diseases
(2021-10-19)Misfolding and accumulation of disease-related proteins are common hallmarks among several neurodegenerative diseases. This phenomenon causes the progressive loss of cognitive and motor functions correlated with specific ... -
The identification and characterization of Mio10 and MINOS1 as novel regulators of mitochondrial inner membrane organization
(2012-06-07)Various mitochondrial inner membrane proteins contribute to the structure of cristae membranes and to the overall dynamic morphology of the inner membrane. These proteins include the F1FOATPase dimers, which contribute to ... -
The interplay between α-synuclein and Rab GTPases: Insights into the molecular basis of synucleinopathies
(2015-03-24)Ageing related diseases become of ever more significance with the demographic shift currently prevalent in society. Dementia and movement disorders impact individuals and families alike, as the progressive decline in ... -
The Mediator kinase module: structural and functional studies in transcription regulation
(2020-10-15)Transcription of protein-coding genes occurs when RNA polymerase II (RNAPII) and general transcription factors (GTFs) assemble at genomic promoter regions, forming a transcription pre-initiation complex (PIC), which ... -
α-synuclein disrupts neuron network rhythmic activity when overexpressed in cultured neurons
(2021-03-09)Synucleinopathies are associated with a generalized slowing of the brain’s rhythmic activity, which co-occurs with dementia and has been connected with increased levels of α synuclein in both patients and test animals. In ...