Differenzierung von Escherichia coli und Pseudomonas aeruginosa mittels Ionenmobilitätsspektrometrie

Göpel, Julia

dc.contributor.advisor	Kunze-Szikszay, Nils PD Dr.
dc.contributor.author	Göpel, Julia
dc.date.accessioned	2022-02-03T08:58:53Z
dc.date.available	2022-02-17T00:50:07Z
dc.date.issued	2022-02-03
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/13856
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-27
dc.language.iso	deu	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	610	de
dc.title	Differenzierung von Escherichia coli und Pseudomonas aeruginosa mittels Ionenmobilitätsspektrometrie	de
dc.type	doctoralThesis	de
dc.title.translated	Differentiation of Escherichia coli and Pseudomonas aeruginosa using ion mobility spectrometry	de
dc.contributor.referee	Perl, Thorsten PD Dr.
dc.date.examination	2022-02-09	de
dc.description.abstracteng	A quick and reliable alternative technology to standard microbiological testing could be the detection of volatile organic compounds (VOC) using multi-capillary column-ion mobility spectrometry (MCC-IMS). The approach to differentiate bacteria and fungi has already been described before. Using a complex fluid medium we first analysed the growth of two human pathogenic reference strains of Escherichia coli and Pseudomonas aeruginosa and generated growth curves. Knowing the time line of the different growth phases we analysed the headspace over the two bacteria at four given times to determine if it is possible to differentiate between the two bacteria and how the VOCs develop over the time. Secondary we analysed clinical samples in order to see whether the the results of the reference strains could be transferred to other strains of the same species. Overall we found 19 VOCs that occurred in our measurements. Most of them had their peak in the late logarithmic or static growth phases. There were 13 VOCs that only appeared over one species, so these VOCs made it possible to differentiate between the two bacteria. There were six VOCs changing only over Escherichia coli and seven VOCs changing only over Pseudomonas aeruginosa. There were no differences in the occurrence of VOCs over the reference strain compared with the clinical isolates of one species.	de
dc.contributor.coReferee	Weig, Michael PD Dr.
dc.subject.eng	Volatile organic compound	de
dc.subject.eng	VOC	de
dc.subject.eng	IMS	de
dc.subject.eng	MCC-IMS	de
dc.subject.eng	Ion mobility spectrometry	de
dc.subject.eng	Multi-capillary column	de
dc.subject.eng	Escherichia coli	de
dc.subject.eng	Pseudomonas aeruginosa	de
dc.subject.eng	Growth curve	de
dc.subject.eng	Clinical isolates	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-13856-5
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	:Medizinische Mikrobiologie / Medizinische Virologie / Medizinische Mykologie / Infektionskrankheiten / Hygiene / Impfung / Parasitologie / Tropenmedizin - Allgemein- und Gesamtdarstellungen (PPN619875356)	de
dc.description.embargoed	2022-02-17	de
dc.identifier.ppn	1794694528

Files in this item

Name:Abgabe-Exemplar.pdf

Size:4.066Mb

Format:PDF

View/Open

Name:: Abgabe-Exemplar.pdf
Size:: 4.066Mb
Format:: PDF

View/Open

This item appears in the following Collection(s)

Human- und Zahnmedizin [2872]

Show simple item record