Proteomic analysis of synaptic dysfunction in the hippocampus of late-stage Parkinson´s Disease
von Carmina Carelia Warth Pérez Arias
Datum der mündl. Prüfung:2021-09-16
Betreuer:Prof. Dr. Paul Lingor
Gutachter:Prof. Dr. Silvio Rizzoli
Gutachter:Prof. Dr. Tiago Fleming Outeiro
EnglischThe present study focussed on assessing changes in the hippocampus as a result of late-stage Parkinson´s disease. Human post-mortem hippocampal samples were used to address these changes, quantifying significant alterations in protein abundance in Parkinson´s disease versus controls. Proteomic data sets were gathered from whole tissue lysates as well as enriched synaptosomal fractions and characterized by means of protein-protein interaction networks, functional annotation analyses and network analyses of protein expression. In addition, the broad proteomic data sets were narrowed down to a single protein of interest, integrating the proteomic characterisation with known literature, placing special interest on the involvement in neurodegenerative diseases and synaptic dysfunction. In summary, this study was able to identify changes in protein expression levels in the hippocampus of Parkinson´s disease patients, characterize the functional interactions and biological relevance of these dysregulated proteins and address in further detail the implication of the dysregulation of a selected protein of interest in a neuronal network.
Keywords: Parkinson´s disease, proteomics, data integration, synaptic dysfunction