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Tumor invasion through 3D engineered basement membrane

dc.contributor.advisorSchilling, Arndt Prof. Dr
dc.contributor.authorZhao, Zhiming
dc.rightsAttribution 4.0 International*
dc.titleTumor invasion through 3D engineered basement membranede
dc.contributor.refereeSchilling, Arndt Prof. Dr
dc.description.abstractengTumor metastasis is the most important reason of cancer-related death. The progress of tumor metastasis includes intravasation, survival in the circulation, extravasation and col- onization. In the process of metastasis intravasation, the basement membrane is the first important barrier. Therefore, engineering a basement membrane in 3D structure might provide a novel 3D model of tumor metastasis, leading to a better understanding of tumor intravasation. In this thesis, a novel artificial engineered basement membrane (EBM) was fabricated by gelation of Matrigel matrix and PEGDA 20,000 under UV exposure. The function of this EBM was investigated by MDA-MB-231 and MCF-7 cell invasion in xCELLigence. MDA-MB-231 cells invaded through the membrane successfully but MCF-7 cells failed, showing that the EBM can differentiate between metastatic and non-metastatic tumor cells. The invasion process was significantly influenced by shikonin and irradiation, showing that the EBM allows to study tumor related treatments. In summary, this thesis established a novel 3D in vitro tumor metastasis model which may serve as a platform for drug screening and study of tumor
dc.contributor.coRefereeWouters, Fred Prof. Dr.
dc.subject.gertumor invasionde
dc.subject.gerbasement membranede
dc.subject.ger3d printingde
dc.subject.engtumor invasionde
dc.subject.engbasement membranede
dc.subject.eng3d printingde
dc.affiliation.instituteMedizinische Fakultätde
dc.subject.gokfullOrthopädie (PPN619876204)de

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