Tumor invasion through 3D engineered basement membrane

Zhao, Zhiming

dc.contributor.advisor	Schilling, Arndt Prof. Dr
dc.contributor.author	Zhao, Zhiming
dc.date.accessioned	2022-04-14T12:01:15Z
dc.date.available	2022-05-04T00:50:16Z
dc.date.issued	2022-04-14
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/13994
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-9180
dc.language.iso	eng	de
dc.rights	Attribution 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject.ddc	610	de
dc.title	Tumor invasion through 3D engineered basement membrane	de
dc.type	doctoralThesis	de
dc.contributor.referee	Schilling, Arndt Prof. Dr
dc.date.examination	2022-04-28	de
dc.description.abstracteng	Tumor metastasis is the most important reason of cancer-related death. The progress of tumor metastasis includes intravasation, survival in the circulation, extravasation and col- onization. In the process of metastasis intravasation, the basement membrane is the first important barrier. Therefore, engineering a basement membrane in 3D structure might provide a novel 3D model of tumor metastasis, leading to a better understanding of tumor intravasation. In this thesis, a novel artificial engineered basement membrane (EBM) was fabricated by gelation of Matrigel matrix and PEGDA 20,000 under UV exposure. The function of this EBM was investigated by MDA-MB-231 and MCF-7 cell invasion in xCELLigence. MDA-MB-231 cells invaded through the membrane successfully but MCF-7 cells failed, showing that the EBM can differentiate between metastatic and non-metastatic tumor cells. The invasion process was significantly influenced by shikonin and irradiation, showing that the EBM allows to study tumor related treatments. In summary, this thesis established a novel 3D in vitro tumor metastasis model which may serve as a platform for drug screening and study of tumor metastasis.	de
dc.contributor.coReferee	Wouters, Fred Prof. Dr.
dc.subject.ger	tumor invasion	de
dc.subject.ger	basement membrane	de
dc.subject.ger	irradiation	de
dc.subject.ger	3d printing	de
dc.subject.eng	tumor invasion	de
dc.subject.eng	basement membrane	de
dc.subject.eng	irradiation	de
dc.subject.eng	3d printing	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-13994-2
dc.affiliation.institute	Medizinische Fakultät	de
dc.subject.gokfull	Orthopädie (PPN619876204)	de
dc.description.embargoed	2022-05-04	de
dc.identifier.ppn	1799695824

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