| dc.contributor.advisor | Thoms, Sven Prof. Dr. | |
| dc.contributor.author | Sargsyan, Yelena | |
| dc.date.accessioned | 2022-04-26T12:04:29Z | |
| dc.date.available | 2024-01-20T00:51:14Z | |
| dc.date.issued | 2022-04-26 | |
| dc.identifier.uri | http://resolver.sub.uni-goettingen.de/purl?ediss-11858/14012 | |
| dc.identifier.uri | http://dx.doi.org/10.53846/goediss-9103 | |
| dc.language.iso | eng | de |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject.ddc | 610 | |
| dc.title | Peroxisomal calcium handling and homeostasis | de |
| dc.type | doctoralThesis | de |
| dc.contributor.referee | Thoms, Sven Prof. Dr. | |
| dc.date.examination | 2022-01-21 | de |
| dc.description.abstracteng | Peroxisomes are ubiquitous cellular organelles in eukaryotes essential for metabolism. The close localization of peroxisomes with the sarcoplasmic reticulum and T-tubules is known from electron micrographs of rodent hearts. This association of peroxisomes with sites of excitation-contraction coupling suggests a peroxisomal role in calcium handling. However, there is little known about peroxisomal calcium and previous studies brought up extensive contradictions.
This work addresses peroxisomal Ca2+ handling in non-excitable HeLa cells, and in excitable cells represented by cardiomyocytes.
Using both models, it is demonstrated that peroxisomal Ca2+ rises in dependance of cytosolic Ca2+ from a steady state of 600 nM to 2.4 µM. It is also shown that over 80% of peroxisomes in cardiomyocytes are in contact with ryanodine receptors and that cardiac peroxisomes take up Ca2+ when intracellular Ca2+ stores are depleted. These results suggest that peroxisomes in cardiomyocytes may contribute to an effective excitation-contraction process. Further, we studied the mechanisms of Ca2+ entry to peroxisomes.
Taken together, this work provides the first evidence of peroxisomal Ca2+ handling taking place in cardiomyocytes and that several mechanisms to interact with it exist. Modulation of peroxisomal Ca2+ homeostasis may have future clinical application in cases of impaired cellular Ca2+ handling, such as certain types of arrhythmias. | de |
| dc.contributor.coReferee | Rizzoli, Silvio Prof. Dr. | |
| dc.subject.eng | peroxisomes, calcium | de |
| dc.identifier.urn | urn:nbn:de:gbv:7-ediss-14012-4 | |
| dc.affiliation.institute | Medizinische Fakultät | |
| dc.subject.gokfull | Medizin (PPN619874732) | de |
| dc.description.embargoed | 2024-01-20 | de |
| dc.identifier.ppn | 1800458142 | |