Probing SNARE-Induced Membrane Fusion Using Peptidic Model Fusogens

by Pirajeev Selvachandran
Doctoral thesis
Date of Examination:2021-12-13
Date of issue:2022-11-10
Advisor:Prof. Dr. Ulf Diederichsen
Referee:Prof. Dr. Claudia Steinem
Referee:Prof. Dr. Kai Tittmann
Persistent Address: http://dx.doi.org/10.53846/goediss-9548

 

 

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Abstract

English

In this thesis, SNARE analogues with artificial recognition units made of either coiled-coil sequences or peptide nucleic acid (PNA) oligomers were synthesized. By applying FRET-based liposome fusion assays, the fusogenic character of SNARE analogues were investigated. The extent of liposome fusion were controllable by the type of the recognition units. Therefore, structural changes of these specific recognition units were applied in order to obtain a detailed insight of the liposome fusion process.
Keywords: High-performance liquid chromatography (HPLC); Solid-phase peptide synthesis (SPPS); SNARE-mimetics; Liposome fusion assays
 
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