Probing SNARE-Induced Membrane Fusion Using Peptidic Model Fusogens
von Pirajeev Selvachandran
Datum der mündl. Prüfung:2021-12-13
Erschienen:2022-11-10
Betreuer:Prof. Dr. Ulf Diederichsen
Gutachter:Prof. Dr. Claudia Steinem
Gutachter:Prof. Dr. Kai Tittmann
Dateien
Name:SUB Dissertation Pirajeev_Selvachandran.pdf
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Format:PDF
Zusammenfassung
Englisch
In this thesis, SNARE analogues with artificial recognition units made of either coiled-coil sequences or peptide nucleic acid (PNA) oligomers were synthesized. By applying FRET-based liposome fusion assays, the fusogenic character of SNARE analogues were investigated. The extent of liposome fusion were controllable by the type of the recognition units. Therefore, structural changes of these specific recognition units were applied in order to obtain a detailed insight of the liposome fusion process.
Keywords: High-performance liquid chromatography (HPLC); Solid-phase peptide synthesis (SPPS); SNARE-mimetics; Liposome fusion assays