Factors stimulating germination of Plasmodiophora brassicae resting spores

Wang, Yao

dc.contributor.advisor	von Tiedemann, Andreas Prof. Dr.
dc.contributor.author	Wang, Yao
dc.date.accessioned	2022-12-07T15:36:29Z
dc.date.available	2022-12-14T00:50:09Z
dc.date.issued	2022-12-07
dc.identifier.uri	http://resolver.sub.uni-goettingen.de/purl?ediss-11858/14396
dc.identifier.uri	http://dx.doi.org/10.53846/goediss-9600
dc.format.extent	106 Seiten	de
dc.language.iso	eng	de
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/
dc.subject.ddc	630	de
dc.title	Factors stimulating germination of Plasmodiophora brassicae resting spores	de
dc.type	doctoralThesis	de
dc.contributor.referee	Ludwig-Müller, Jutta Prof. Dr.
dc.date.examination	2021-06-17	de
dc.description.abstracteng	Cruciferous crops have significant economic value as vegetables, edible and industrial oil sources, animal feeds, manure and biofuel. Clubroot caused by Plasmodiophora brassicae, a worldwide spread soil-borne disease seriously restricts the production of cruciferous crops. The major symptom of clubroot is single or multiple root galls that limit the uptake of water and nutrients leading to stunting and considerable losses in yield and quality. The germination of P. brassicae resting spores is a key determinant of infection and disease severity. Thus, identifying biotic and abiotic stimulants for spore germination is imperative for developing novel clubroot control strategies. The accurate assessment of viability and germination rate is the foundation to evaluate the effect of control methods. In this study, we optimized several methods for the assessment of viability and germination rate of P. brassicae resting spores to obtain reliable estimates. Furthermore, our studies showed that pure root exudates collected under sterile conditions cannot trigger resting spore germination, which is contrasted to previous studies. Instead, various bioassays results demonstrated that soil bacteria are essential for P. brassicae resting spore germination. We found that certain carbon sources and nitrate can reshape the initial microbial community to an inducing community leading to the germination of P. brassicae resting spores. The stimulating communities significantly differed in composition and abundance of bacterial taxa compared to the non-stimulating ones. A multi-factorial ‘pathobiome’ model comprising abiotic and biotic factors is proposed to represent the putative plant-microbiome-pathogen interactions associated with breaking spore dormancy of P. brassicae in soil. This study presents novel insights significantly improving our understanding about the interaction of soil microbiota with P. brassicae and its host plant, which may develop novel strategies of clubroot disease control.	de
dc.contributor.coReferee	Dittert, Klaus Prof. Dr.
dc.subject.eng	Clubroot	de
dc.subject.eng	Germination	de
dc.subject.eng	CLSM	de
dc.subject.eng	Disease severity	de
dc.subject.eng	Microbial community	de
dc.subject.eng	Root exudates	de
dc.subject.eng	Pathobiome	de
dc.identifier.urn	urn:nbn:de:gbv:7-ediss-14396-1
dc.affiliation.institute	Fakultät für Agrarwissenschaften	de
dc.subject.gokfull	Land- und Forstwirtschaft (PPN621302791)	de
dc.description.embargoed	2022-12-14	de
dc.identifier.ppn	182674665X
dc.identifier.orcid	0000-0001-6824-8852	de
dc.notes.confirmationsent	Confirmation sent 2022-12-07T15:45:01	de

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