Auswirkungen der Rezeptor-Dimerisierung auf das Calcium-imaging muskarinerger Acetylcholinrezeptoren M1, M3 und M5
von Ilya Edip Zengin
Datum der mündl. Prüfung:2022-12-15
Erschienen:2022-12-15
Betreuer:PD Dr. Marcus Niebert
Gutachter:Prof. Dr. Martin Oppermann
Gutachter:Prof. Dr. Thomas Meyer
Dateien
Name:Dissertation Ilya Zengin finale Version.pdf
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Format:PDF
Zusammenfassung
Englisch
This dissertation focuses on the effects of receptor-dimerization regarding the muscarinic Acetylcholine-receptors M1, M3 and M5. The goal was to evaluate whether the dimerization between homo- or heterologous receptor partners between M1, M3, and M5 have an impact on the following intracellular receptor-signalling. We developed a method to enforce dimerization between two recepor-partners by generating receptors which have attached either an antigen (GFP) or an anti-GFP-antibody. By co-expressing both modified receptors on the cell surface we could ensure a forced dimerization between the two receptor partners due to direct antibody-antigen binding. We interpreted the impact of dimerization by measuring and comparing the intracellular calcium-levels in with live cell calcium-imaging. The results showed, that dimerization between two mAChRs can lead to potential coupling mechanism between the receptors and have an impact on the signalling of those.
Keywords: Dimerization; mAChR; Calcium-imaging