Auswirkungen der Rezeptor-Dimerisierung auf das Calcium-imaging muskarinerger Acetylcholinrezeptoren M1, M3 und M5

by Ilya Edip Zengin
Doctoral thesis
Date of Examination:2022-12-15
Date of issue:2022-12-15
Advisor:PD Dr. Marcus Niebert
Referee:Prof. Dr. Martin Oppermann
Referee:Prof. Dr. Thomas Meyer
Persistent Address: http://dx.doi.org/10.53846/goediss-9614

 

 

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Abstract

English

This dissertation focuses on the effects of receptor-dimerization regarding the muscarinic Acetylcholine-receptors M1, M3 and M5. The goal was to evaluate whether the dimerization between homo- or heterologous receptor partners between M1, M3, and M5 have an impact on the following intracellular receptor-signalling. We developed a method to enforce dimerization between two recepor-partners by generating receptors which have attached either an antigen (GFP) or an anti-GFP-antibody. By co-expressing both modified receptors on the cell surface we could ensure a forced dimerization between the two receptor partners due to direct antibody-antigen binding. We interpreted the impact of dimerization by measuring and comparing the intracellular calcium-levels in with live cell calcium-imaging. The results showed, that dimerization between two mAChRs can lead to potential coupling mechanism between the receptors and have an impact on the signalling of those.
Keywords: Dimerization; mAChR; Calcium-imaging
 
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