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Cotranslational folding of HemK C-terminal domain

dc.contributor.advisorRodnina, Marina Prof. Dr.
dc.contributor.authorDaberger, Jan
dc.date.accessioned2023-03-17T17:49:41Z
dc.date.available2024-02-12T00:51:06Z
dc.date.issued2023-03-17
dc.identifier.urihttp://resolver.sub.uni-goettingen.de/purl?ediss-11858/14580
dc.identifier.urihttp://dx.doi.org/10.53846/goediss-9786
dc.format.extentXX Seitende
dc.language.isoengde
dc.subject.ddc570de
dc.titleCotranslational folding of HemK C-terminal domainde
dc.typedoctoralThesisde
dc.contributor.refereeRodnina, Marina Prof. Dr.
dc.date.examination2023-02-13de
dc.description.abstractengThe correct folding of proteins is essential for the survival of the cell. Despite the importance of the question, it remains largely unclear how proteins, in particular large multidomain proteins with mixed α-helical and β-strand structures, fold cotranslationally. In this study, we chose the C-terminal domain of E. coli enzyme HemK as the model protein to study folding in a fully reconstituted in vitro translation system. Here, we present the folding pathway of the protein step-by-step employing force profile assay using the SecM arrest peptide in combination with time-resolved and steady-state photo-induced electron transfer and Förster resonance energy transfer measurements. This combination allows us to analyze the folding pattern both inside and outside of the polypeptide exit tunnel of the ribosome and to assess the stepwise compaction of the nascent chain. The N-terminal β-hairpin of the C-terminal domain starts to fold inside the polypeptide exit tunnel and undergoes structural rearrangements before emerging from the ribosome. As the nascent peptide emerges from the ribosome, it folds sequentially, starting from the first β-hairpin, which could serve as the folding nucleus for the following C-terminal part adopting a Rossmann fold. The folded domain remains highly dynamic on the ribosome and the final compaction into the native structure occurs after its release from the ribosome.de
dc.contributor.coRefereeEnderlein, Jörg Prof. Dr.
dc.contributor.thirdRefereeStein, Alexander Dr.
dc.contributor.thirdRefereeTittmann, Kai Prof. Dr.
dc.contributor.thirdRefereeLorenz, Sonja Dr.
dc.contributor.thirdRefereeFaesen, Alex Dr.
dc.subject.engCotranslational foldingde
dc.subject.engRibosomede
dc.subject.engIn vitro translationde
dc.identifier.urnurn:nbn:de:gbv:7-ediss-14580-8
dc.affiliation.instituteBiologische Fakultät für Biologie und Psychologiede
dc.subject.gokfullBiologie (PPN619462639)de
dc.description.embargoed2024-02-12de
dc.identifier.ppn1839532939
dc.notes.confirmationsentConfirmation sent 2023-03-20T06:15:01de


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